CRMP2对MCAO大鼠的神经保护作用

项目名称： CRMP2对MCAO大鼠的神经保护作用

项目编号： No.81200900

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 神经系统疾病、精神疾病

项目作者： 王恬竹

作者单位： 重庆医科大学

项目金额： 23万元

中文摘要： 脑梗死是危害人类生命健康的危重病之一，中风患者的神经功能康复是近年来临床医生比较关注的问题，一直缺乏有效治疗手段。本课题旨在积极调动外源性干预手段进行有效的神经保护治疗，最终使受损的神经功能重建。其关键在于寻找或开发一种能够同时阻断多条病理损伤途径的药物，在减少神经元丢失的情况下，还能促进内源性神经元的补充。Collapsin反应调节蛋白2（CRMP2）参与调节了多种神经生理活动，在我们的前期工作中证实了其促进神经修复、轴突再生的作用及相关机制，从而提示其可能具有良好的神经保护潜能。因此，我们拟构建CRMP2的真核表达载体，使脑内CRMP2过度表达，观察其对神经细胞凋亡，凋亡基因/抗凋亡基因的转录，内源性神经干细胞增殖、分化及迁移的作用及可能机制，以及对神经功能的影响。本课题的研究成果将扩展对CRMP2病理生理作用的认识，也为CRMP2作为临床干预靶点提供前期的试验依据。

中文关键词： MCAO/再灌注；CRMP2；凋亡；神经干细胞；神经保护

英文摘要： Cerebral infarction is one of the critical disease harmful to human health life, neurological function recovery after a stroke is a concern of clinical doctors in recent years, lack of effective treatment. This project aims to actively mobilize external intervention for effective neural protection treatment, and finally make the damaged nerve function reconstruction. The key lies in finding or developing a new drug by blocking several pathological pathways, to reduce the loss of neurons and promote the endogenous neurogenesis. Collapsin response mediator protein 2 (CRMP2) participates in a various regulation of neural physiological activities. In our early work it's proved its mechanism on axonal regeneration，suggesting that it may have good neuroprotective potential. Therefore, we are planning to build the CRMP2 eukaryotic expression vector, resulting in excessive CRMP2 expression in brain. Then the effects of CRMP2 on the apoptosis of nerve cells, the transcription of apoptosis/ antiapoptosis genes, the proliferation, differentiation and migration of the endogenous neural stem cell, and the possible mechanism were studied, and the influence on neurological function was evaluated. The research results will be expanded to the understanding of CRMP2 pathophysiological roles, and provide an initial trial data for

英文关键词： MCAO/reperfusion；CRMP2；apoptosis；neural stem cell；neuroprotective