羊栖菜抗衰老多糖级分的筛选及抗衰老机制的初步研究

项目名称： 羊栖菜抗衰老多糖级分的筛选及抗衰老机制的初步研究

项目编号： No.31470430

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 吴明江

作者单位： 温州大学

项目金额： 86万元

中文摘要： 羊栖菜是传统的药食两用海藻，被公认具益寿功效，称为长寿菜，但对其发挥抗衰老作用的物质基础及作用途径仍缺乏认识。申请人的前期工作表明，羊栖菜粗多糖可以激活ARE报告基因的表达，延缓D-半乳糖亚急性衰老小鼠的衰老进程，这暗示着羊栖菜粗多糖中含有抗氧化和抗衰老的有效成分。本项研究将依次通过冷水、热水和碱液浸提法提取粗多糖，再分别用离子交换层析柱和凝胶过滤层析柱进行纯化分级，并利用ARE报告基因法、H2O2促衰老细胞模型和黑腹果蝇模型逐级筛选不同纯化步骤获得的羊栖菜多糖，探明发挥抗衰老作用的多糖级分。随后，在细胞水平和个体水平上分别对Nrf2表达水平、磷酸化修饰模式及其上游蛋白进行研究，探明有效级分发挥抗氧化作用的机制；再结合对黑腹果蝇转录组的研究，初步探讨羊栖菜多糖有效级分发挥抗衰老作用的分子机理。本研究结果将为植物多糖抗衰老有效成分的开发提供借鉴，也为阐明多糖抗衰老的作用机制提供实验依据。

中文关键词： 羊栖菜；多糖；抗衰老；抗氧化

英文摘要： The seaweed, Hizikia fusiformis, is a kind of Chinese traditional medicine, which is well acknowledged as life extension food. However, the components in H. fusiformis which display anti-aging effect and the related mechanisms are unkown. Our previous research showed that crude H. fusiformis polysaccharides activated ARE reporter gene and slowed down the aging process of D-galactose induced aging mice, which suggested that the crude Hizikia fusiformis polysaccharides contained anti-oxidative and anti-aging components. In this project, we propose to separate and purify the H. fusiformis polysaccharides, then screen the fractions with powerful anti-aging effect using ARE reporter gene, H2O2 induced premature senescence cell model and drosophila melanogaster model. Meanwhile, analyze the expression and phosphorylation pattern of transcriptional factor Nrf2 and the change of its upstream proteins at cell level, as well as the transcriptome in the drosophila melanogaster model to investigate the anti-aging mechanism of H. fusiformis polysaccharides. This research study will provide a theoretical knowledge for exploring anti-aging polysaccharides and contribute to understanding the anti-aging mechanism of polysaccharides.

英文关键词： Hizikia fusiformis;Polysaccharide;Anti-aging;Anti-oxidant