油菜素内酯和生物钟协调控制植物生长和免疫抗病平衡的分子机制

项目名称： 油菜素内酯和生物钟协调控制植物生长和免疫抗病平衡的分子机制

项目编号： No.31470376

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 植物学

项目作者： 白明义

作者单位： 山东大学

项目金额： 95万元

中文摘要： 植物生长和免疫的动态平衡是植物增产抗病的关键，如何保证植物在抗病的同时还能增产是国际植物学研究的前沿热点领域。植物生长和免疫抗病受到植物激素和生物钟系统的协同调控，但其分子机制目前还不是很清楚。我们前期的研究工作显示植物甾醇类激素油菜素内酯（BR）通过其下游关键的bHLH转录因子HBI1，与病原菌分泌产生的微生物相关分子（PAMPs）相拮抗，协调控制植物生长和免疫抗病的平衡。我们最新的研究结果显示HBI1能与生物钟关键调控蛋白HIC1形成复合体共同调控基因表达。因此在本项目中我们将通过研究HBI1与HIC1的互作机理，及HBI1和HIC1调控的基因网络来探讨HBI1和HIC1在BR信号转导、生物钟调控、植物生长和免疫抗病中的功能，来逐步阐明植物在正确的时间准确调控植物生长和免疫抗病平衡的分子机制，为我们下一步高产抗病育种提供扎实的理论基础和技术保障。

中文关键词： 油菜素内酯；生物钟；植物生长；植物免疫；转录调控

英文摘要： The trade-off between growth and immunity is crucial for high yield and disease-resistance of crops. This trade-off is believed to require complex interactions between plant hormones, circadian rhythm and environmental signals. However, the mechanism underlying growth-immunity balance has remained elusive. The bHLH transcription factor HBI1, as a key component of brassinosteroid (BR) signaling transduction, has been reported to play an important role in regulating the dynamic balance of plant growth and immunity. Recently our research results showed that HBI1 interacted with the key regulator of circadian clock, HIC1 to regulate the gene expression, suggesting HBI1 and HIC1 maybe integrate BR and circadian rhythm to control plant growth and immunity. Here we want to analysis the HIC1 binding and regulated genes in Arabidopsis genome, construct the HBI1- and HIC1-regulated gene network, and functional study of HBI1 and HIC1 in BR signal transduction, circadian oscillation, plant growth and immunity. These results will help us to clarify the mechanism in which BR and circadian cross talk to regulate the trade-off between growth and immunity, and provide some new tools for breeding disease-resistance high-yield crops.

英文关键词： Brassinosteroid;Circadian clock;Plant growth;Plant immunity;Transcriptional regulation