影响大豆低植酸突变体籽粒活力的因素分析及其分子作用机制的研究

项目名称： 影响大豆低植酸突变体籽粒活力的因素分析及其分子作用机制的研究

项目编号： No.31271754

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 袁凤杰

作者单位： 浙江省农业科学院

项目金额： 80万元

中文摘要： 植酸是一种抗营养因子，降低大豆植酸含量是大豆品质改良的重要内容之一。然而植酸及其代谢途径中的肌醇又是重要的抗氧化因子和信号分子，在种子萌发、幼苗生长、籽粒休眠和抗逆性等方面具有重要的功用。可见，大豆籽粒活力与植酸密切相关。研究表明不同突变基因对籽粒活力影响不同，含有相同突变基因的低植酸突变体，籽粒活力也不完全一致。尽管在拟南芥、玉米等作物的研究中，明确了植酸在抗氧化和激素调节方面对籽粒活力产生影响，但缺乏分子水平上的深入探讨；低植酸突变基因对大豆籽粒活力影响机制的研究尚属空白。本研究应用自主创造的低植酸突变体，以组织形态学、代谢组学、转录组学和蛋白组学的研究方法，探讨低植酸大豆对籽粒活力的影响因素，研究不同突变基因对籽粒活力的作用模式，在RNA和蛋白表达水平上发掘提高低植酸大豆籽粒活力相关基因，研究基因互作模式，提出改良低植酸大豆籽粒活力的方法和途径，为低植酸作物籽粒活力改良奠定理论基础。

中文关键词： 大豆；低植酸；籽粒活力；因子；分子机制

英文摘要： Phytic acid is an antinutritional factor in soybean seeds, lowering the content of phytate is very important to improve the nutritional quality of soybean seed. Otherwise phytic acid has crucial antioxidative function and regulates some decisive pathway as a singalling molecular on seed germination, dormancy,seedling growth and resistant stress. Evidence from a maize low phytic acid (lpa) mutant showed that phytic acid prevented oxidative stress in seeds; MIPS1 gene involved in pathway of phytic acid synthesis was also required for suppression of cell death; A defective of MRP family in bean and Arabidopsis lpa mutants altered ABA sensitivity. Researches above indicated that there could be a significant relationship between phytic aicd content and seed vigor. Although some lpa soybean mutants with lower seed germination and field emergence rates have been reported, the mechansim of lowering seed vigor in these mutants has not been studied. On previous research, we got some lpa mutants with different seed vigor and different mutation genes, and in this study, we use these different low phytic acid mutants to evaluate the main factors affecting lpa soybean seeds vigor, and study their RNA and protein expression patterns during seed developing and germination stages, clone some new genes which involved in the pathw

英文关键词： soybean；low phytate；seeds vigor；factors；molecular mechanism