柑桔类病毒互作以及寄主响应的分子机理研究

项目名称： 柑桔类病毒互作以及寄主响应的分子机理研究

项目编号： No.31501611

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 曹孟籍

作者单位： 中国农业科学院柑桔研究所

项目金额： 18万元

中文摘要： 柑桔类病毒是在世界范围内广泛发生并可造成严重经济损失的柑桔病原物。前期研究发现在中国的柑桔产区，部分与裂皮病相类似的病害是由非CEVd的多种柑桔类病毒复合侵染造成的；苹果锈果类病毒属内的柑桔类病毒具有典型的协生作用；CBCVd对CEVd存在拮抗作用。本课题拟通过类病毒侵染性克隆的构建和实时荧光定量RT-PCR等技术，监控柑桔类病毒单独/复合侵染后积累量的变化。借助于小RNA深度测序及分析，明确柑桔类病毒单独/复合侵染后是否诱导/抑制柑桔内源小RNA通路。通过柑桔基因芯片、分子杂交等技术分析寄主基因表达差异，解释这些小RNA的功能。最终从基因沉默理论水平上解析柑桔类病毒之间拮抗作用和协生作用的分子机理。本项目的开展可为预防柑桔类病毒病害在我国发生流行提供理论依据和技术支撑，并可望为柑桔裂皮病及其它类病毒病害的防控提供新的思路。

中文关键词： 果树病毒病；基因沉默；复合侵染；小RNA；致病机制

英文摘要： Citrus viroid is one of the most important citrus pathogen in the world. The previous study showed that certain exocortis-like diseases in China were caused by some combination of citrus viroids except CEVd. Typical synergism between citrus viroids from Apscaviroid genus and antagonism between CBCVd and CEVd were also observed. In this study, viroid titer changes after complex infection will be tested through viroid infection clone and real-time RT-PCR. In addition, with the analysis of deep sequencing data of small RNA, we will verify whether the endogenous small RNA would be induced or depressed by viroid infection. Finally, the function of these small RNAs will be validated by citrus microarray and molecular hybridization. All of these will help us to explain the molecular mechanism of interaction between citrus viroids and establish the theoretical and technical foundation of citrus viroid pathogenesis, as well as providing new ideas for controlling of exocortis or other viroid diseases.

英文关键词： Fruit tree virus disease;Gene silence;Multiplex infection;Small RNA;Pathogenesis