MIR396对水稻小穗发育的调控机理

项目名称： MIR396对水稻小穗发育的调控机理

项目编号： No.31270331

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 徐云远

作者单位： 中国科学院植物研究所

项目金额： 80万元

中文摘要： 水稻花器官颖片的开闭控制柱头外露，而这一过程受其发育状态的直接影响。虽然水稻颖片发育相关基因已有报道，但多基因参与的调控机制知之甚少。揭示颖片开闭机制在理论上和杂交制种中具有重要意义。本项目将基于本实验室前期的miR396超表达水稻表现出护颖明显增长、颖壳开裂的稳定表型，重点研究该miR396与预测的可能靶基因OGP6及其调控基因所组成的分子模块对颖片发育的调控机制。研究内容包括这些基因超表达株系及其突变体的遗传学分析、转基因植物与突变体颖片发育的细胞学分析、OGP基因家族表达模式与生化特性，OGP互作蛋白筛选与鉴定、以及转基因植物颖片发育的生理调节。科学目标是揭示颖片发育基因调控网络的分子机制，特色是系统研究多基因协同控制开颖表型的分子网络。前期研究结果初步构筑了这些基因作用模式的基本框架，有望揭示多基因网络控制颖片发育机制。

中文关键词： 水稻；转录因子；OsmiR396d；靶基因；

英文摘要： Stigma exsertion is regulated by hull closure which is determined by the development state of floral organs. Although the development of rice floral organs have been reported, the regulation mechanism mediated by multiple genes is poorly understood.It is important in theory and hybrid seed production to reveal the mechanism of hull closure. OsMIR396 gene was cloned previously in our group and overexpressed in rice. Several transgenic rice lines were selected. The phenotypical analysis indicated that overexpressed lines showed longer glume and opened-hull. Based on the bioinformatic prediction, we have screened one of the miR396 target gene OGP6. This project will focus on how the module consisting of miR396, OGP6 and its target gene to regulate the development of lemma and palea. The specific research include: (1) the expression pattern of OsMIR396 and OGP6 will be checked to confirm their spatial and temporal distribution, (2) phenotypical analysis of OsmiR396- and OGP6-overexpressed transgenic lines and ogp6 mutant line, (3) the screening of OGP intereaction proteins by Y2H and their biochemical characterization and screening of downstream regulated genes of OGP6, (4) the regulation of transgenes on glume developmented. The scientific objectives is to reveal the molecular mechanism of the glume development and

英文关键词： rice (Oryza sativa)；transcription factor；OsmiR396d；target gene；