超支化聚合物用于材料抗蛋白非特异性吸附的研究

项目名称： 超支化聚合物用于材料抗蛋白非特异性吸附的研究

项目编号： No.51203089

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 有机高分子材料学科

项目作者： 何琳

作者单位： 上海交通大学

项目金额： 25万元

中文摘要： 当生物医学材料与血液接触时，血液中的蛋白质会非特异性吸附在材料表面，导致了血小板的黏附和血栓的形成。抗蛋白质非特异性吸附材料是近年来被广泛研究的新一代生物医学材料，它通过与水的强烈相互作用防止了蛋白质分子的非特异性吸附，从而避免了后续的一连串生化反应，达到防止凝血、降低排异等目的。本项目针对现有抗蛋白质非特异性吸附材料的局限性，提出利用亲水性高度支化聚合物修饰基体表面降低蛋白质的非特异性吸附。高度支化聚合物溶解性能优异，且表面具有大量官能团，可以根据实际需要进行表面改性提高材料抗蛋白吸附性能，有望得到性能优异的抗蛋白质非特异性吸附生物医学材料。高度支化聚合物的表面官能团还可用于耦合抗体，由于与基体偶联的亲水高度支化聚合物具有超低蛋白吸附背景，所以该材料还能实现血浆中特定蛋白的特异性检测。

中文关键词： 蛋白吸附；超支化聚合物；细胞黏附；纳米金棒；壳聚糖

英文摘要： When the biomaterials are contacted with biological fluids, such as blood or other body fluids, nonspecific protein adsorption is known to be the first event that occurs on the material surfaces. That will lead to a cascaded of host responses including blood coagulation, thrombus formation, platelet activation, cell and bacterial infection, and other undesirable responses. The material which resists the nonspecific protein adsorption has attracted scientist's great interest in recent years.These materials interact with water strongly to prevent the nonspecific adsorption of protein molecules. Thus the sebsequent a series of biochemical reactions can be attenuated, reaching the purpose of preventing clotting and reducing the rejection and so on. In this project, the substrate surface will be modified by hydrophilic highly branched polymers to reduce nonspecific adsorption of the protein.Highly branched polymer has properties of excellent solubility and a large number of functional groups, that makes it possible to modify the surface of material according to actual needs for improving the material resistance to protein adsorption. It is expected to obtain the biomedical material with the excellent performance of resisting protein nonspecific adsorption. In addition, a large number of surface functional groups of

英文关键词： protein adsorption；hyperbranched polymer；cell adhesion；Au nanorod；chitosan