叶酸受体靶向的近红外荧光共轭聚合物纳米材料的构建及应用

项目名称： 叶酸受体靶向的近红外荧光共轭聚合物纳米材料的构建及应用

项目编号： No.21374059

项目类型： 面上项目

立项/批准年度： 2014

项目学科： 高分子科学

项目作者： 熊丽琴

作者单位： 上海交通大学

项目金额： 40万元

中文摘要： 荧光共轭聚合物（FCP）光稳定性好、毒性小，是新一代荧光纳米探针的发展方向。但其发射波长在可见光范围内，限制了其在活体成像中的应用。发展近红外光（NIR）发射的FCP纳米探针是一个关键难点。针对于此，申请人前期研究设计合成了发射波长在778nm、NIR量子产率为0.09的NIRFCP纳米探针，耦联RGD肽后，可靶向成像高表达整合素αvβ3受体的肿瘤。但作为靶向试剂，该探针在肝脾皮肤的非特异摄取阻碍了其对较小肿瘤的靶向成像。如何减少探针的体内非特异性摄取是一个亟待解决的关键问题。本项目拟在已有工作基础上，构建叶酸受体靶向的NIRFCP纳米探针，并探讨粒径大小、表面电荷、以及PEG链长度对NIRFCP纳米探针的体内非特异性摄取的影响,旨在提高NIRFCP纳米探针在肿瘤部位的摄取量，拓展NIRFCP纳米探针在肿瘤分子影像领域中的应用。本项目的结果将为构建更为有效的肿瘤靶向荧光纳米探针提供新思路。

中文关键词： 近红外；荧光共轭聚合物；纳米材料；分子成像；叶酸受体

英文摘要： Fluorescent conjugated polymers (FCP) have emerged as a novel class of promising fluorescent probes for bioimaging with high fluorescence, good photostability and low cytotoxicity. However, as most of the reported FCP nanoparticles have low emission intensity in the near-infrared (NIR) region, it greatly restricted their application in in vivo imaging. Exploration of FCP nanoparticles containing energy deficient units with NIR emission signature in the polymer backbones can be a promising strategy to fabricate FCP nanoparticles with bright NIR emission. Based on this, we have developed NIRFCP nanoparticles with emission at 778 nm and the quantum yield of the 778nm emission in water was around 0.09. And this nanoparticle can be further used for targeted imaging integrin αvβ3 receptor high-expressed tumors in vivo after conjugation with RGD peptide. As a target agent, the non-specific uptake in the liver, spleen and skin greatly restricted its application in targeted imaging smaller size tumors. Therefore, how to reduce the non-specific uptake of probes is an urgent problem needsed to be solved. In this project, based on our previous study, we purpose to improve the tumor uptake of folate conjugated NIRFCP nanoparticles by investigating the effects of particle size, surface charge, and PEG linkage length on the in

英文关键词： near-infrared；fluorescent conjugated polymer；nanoparticle；molecular imaging；folate receptor