基于金纳米粒子LSPR效应的生物免疫传感研究

项目名称： 基于金纳米粒子LSPR效应的生物免疫传感研究

项目编号： No.61275153

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 无线电电子学、电信技术

项目作者： 周骏

作者单位： 宁波大学

项目金额： 93万元

中文摘要： 传染性疾病对于公众健康和国家安全具有巨大威胁，传染病的快速诊断是防治和控制大规模传染病流行的关键，迫切需要研发传染病病原体早期筛查的生物检测技术和仪器。另一方面，由于纳米材料的优异性能，特别是贵金属纳米粒子的局域表面等离子体共振(LSPR)效应，在生物传感领域的应用成为新的研究热点。本项目通过研究金纳米粒子的结构（大小、形状和组成）及其介电性质（整体和局域介质环境）等特性参数对传染病病原体的LSPR消光光谱形状和特征消光峰位移的影响，结合现代生物免疫学原理，深入理解LSPR生物免疫传感的物理机制。同时，优化金纳米粒子制备工艺，利用电子束刻蚀和Anopore膜过滤沉积组装制备生物芯片，搭建LSPR光谱高分辨率测量系统，实现高敏感度生物分子免疫特异性的检测和分析。本项目开展金纳米粒子LSPR效应的理论研究和LSPR生物免疫传感的实验研究，为新型生物光学传感器件的开发奠定重要的理论和技术基础。

中文关键词： 金纳米结构；局域表面等离子体共振；表面增强拉曼散射；免疫检测；生物传感

英文摘要： Infectious diseases have an enormous threat for public health and state security, the fast diagnoses is a key for the prevention and control of a large-scale epidemic so that to develop the bio-detecting technology and equipment is a urgent need for the application of the early filtration of infectious disease pathogen. On the other hand, as the excellent performance of nano-material, especialy, the localized surface plasmon resonance (LSPR) effect of the precious metals has been become a new and hot research point in the biological sensing field. In this project, combining the principle of moden immunology, the affects of the characteristic parameters of gold nanoparticle such as the structure (size, shape and component) and the dielectric properties (in bulk and surface environment) to the shape of LSPR extinction spectra and the displacement of characteristic extinction peak of infectious disease pathogen are researched for thorough understanding the physical mechanism of LSPR sensing with biological immune. At the same time, the preparation technic process of gold nanoparticle is optimized, the biochips are fabricated by Electron beam lithography and the deposition assemble of Anopore film filtering technology, and the measurement system of high-resolution LSPR spectral is builded up to implement the highly

英文关键词： Gold nanostructure；Localized surface plasmon resonance (LSPR)；Surface-enhanced Raman scattering (SERS)；Immunity；Biosensing