枯草杆菌芽孢衣蛋白自组装的研究

项目名称： 枯草杆菌芽孢衣蛋白自组装的研究

项目编号： No.21275140

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 唐纪琳

作者单位： 中国科学院长春应用化学研究所

项目金额： 80万元

中文摘要： 枯草杆菌的芽孢衣是生物分子自组装方面的特例，它是由约70 种蛋白通过弱键和弱特异性相互作用形成规则的层状结构，这些规则排列的蛋白赋予芽孢抵抗外界热、干旱、辐射、有毒化学物质和酶分解等属性。本项目拟以芽孢衣形态发生蛋白如SpoIVA、SpoVID和CotE，还有起着中间体作用的芽孢衣形态发生蛋白如SafA、CotH 和CotO，及其它芽孢衣蛋白如CotV、CotW、CotY 和CotZ 为主要研究对象，用原子力显微镜在单分子水平上研究芽孢衣蛋白间的相互作用及动力学过程，模拟并高分辨监控芽孢衣蛋白的自组装过程，同时结合紫外可见光谱、圆二色谱、动态光散射和表面等离子共振等技术深入系统地探索芽孢衣蛋白自组装过程及调控规律，实现单一或复合芽孢衣蛋白在生物体外的可控自组装，建立和发展新的蛋白自组装结构和体系，构筑对外界的有毒物质或不良环境有抵抗力功能的自组装体系。

中文关键词： 枯草芽孢衣蛋白；自组装；原子力显微镜；单分子识别；

英文摘要： The spore coat of Bacillus subtilis endospore represents unique example of macromolecular assemblies. It is composed of at least 70 different proteins that via weak bond and weak specific interaction arrange into a complex multilayered structure. This highly ordered structure endows the spore with ability to defend itself against extreme environmental conditions, including heat, drought, UV radiation, toxic chemicals and lysozyme treatment, etc. Within the scope of this project will address the fundamental questions about how the spore coat is assembled. This proposal focus on the spore coat proteins with crucial morphogenetic role in the spore coat formation SpoIVA, SpoVID, and CotE, and on the proteins with intermediate morphogenetic role as SafA, CotH and CotO, and also on some outer spore coat proteins CotV, CotW, CotY, CotZ. The interactions and dynamic processes among spore coat proteins will be studied by Atomic force microscopy (AFM) at the single molecule level. The high-resolution AFM imaging is used to monitoring the assembly pathway and dynamics of spore coat proteins in vitro. Combined with UV-VIS spectrum and circular dichroic spectroscopy, dynamic light scattering and surface plasmon resonance, we want to establish a new model for spore coat self-assembly process. The attention will be also brough

英文关键词： Bacillus subtilis spore coat proteins；Self-assembly；Atomic foce microscopy；Single Molecular recognition；