Tim-3影响肾癌患者细胞免疫功能调节机制的研究

项目名称： Tim-3影响肾癌患者细胞免疫功能调节机制的研究

项目编号： No.81202019

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 肿瘤学2

项目作者： 蔡晨

作者单位： 中国人民解放军第二军医大学

项目金额： 23万元

中文摘要： 肾癌是泌尿系统最常见的肿瘤之一，其发生发展机制目前受广泛关注。近年国内外研究证实Tim-3在肿瘤患者的细胞免疫调节中具有重要的作用。Tim-3及其参与的肿瘤免疫调节机制在肾癌中的作用目前尚无相关报道。我们前期研究发现Tim-3的基因多态性与肾癌发病有一定相关性，肾癌患者外周血CD4+T 细胞和CD+8 T细胞中Tim-3+细胞所占比例均有明显增加，但其功能与作用机制未明。我们推断Tim-3可能通过影响肾癌患者的细胞免疫功能影响疾病进程。本课题拟研究不同肾癌分期患者体内Tim-3的分布情况，通过阻断或激活Tim-3/galectin-9等试验，分析Tim-3与不同分期肾癌患者Th1、Th2、树突细胞、CD4+T、CD8+Treg等免疫细胞功能的关系，探讨Tim-3参与细胞免疫调节的作用及机制，从而为肾癌的生物治疗提供新的治疗靶点和理论依据。

中文关键词： 肾细胞癌；Tim-3；免疫反应；；

英文摘要： Kidney cancer is one of the most frequent cancer in urinary system, and the mechanism of its generation and development had caused people to pay wide attention. Recently domestic and international researches confirmed that Tim-3 had very important effects in cell immunoloregulation of tumor patients. The effects of Tim-3 and the tumor immunoregulation mechanism in kidney cancer were not reported yet. Our early researches indicated the polymorphism of Tim-3 gene had some relationship to the pathogenesy of kidney cancer,and we also discovered that the ratio of Tim-3+ cell in CD4+T lymphocyte and CD+8 T lymphocyte of kidney cancer patients had obviously increased ,compared to the ratio in health crowd. But the function and the action mechanism were still unknown. So we ratiocinate that Tim-3 possible influence the disease process by regulating cellular immune function in kidney cancer patients. We plan to research the distribution of Tim-3 , analysis the relation of Tim-3 to immunocyte(such as Th1,Th2, dendritic cells and CD4+、CD8+Treg) by some experiments(such as block or active Tim-3/galectin-9), and investigate the regulative effect and mechanism of Tim-3 in kidney cancer cytoimmunity in different stages of kidney cancer patients. We wish provide a new therapy target and theoretical basis for the biological the

英文关键词： renal cell carcinoma；Tim-3；immune response；；