基于β-葡聚糖受体Dectin-1的黑灵芝多糖免疫调节作用机制

项目名称： 基于β-葡聚糖受体Dectin-1的黑灵芝多糖免疫调节作用机制

项目编号： No.31201326

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 膳食与营养

项目作者： 李文娟

作者单位： 南昌大学

项目金额： 26万元

中文摘要： 多糖研究是当今生命科学领域研究热点与难点。本项目组已从黑灵芝中纯化出一种β-葡聚糖结构特征的多糖；进一步证实该多糖能通过增强宿主免疫功能发挥抗肿瘤作用，但其抗肿瘤免疫机理目前尚不清楚。最新发现的β-葡聚糖受体Dectin-1对β-葡聚糖的免疫识别为研究免疫反应开辟了全新领域，已成为新的设计靶点，并将推动以β-葡聚糖为基础的抗肿瘤作用研究。为了阐明Dectin-1能否识别黑灵芝多糖的β-葡聚糖结构，介导黑灵芝多糖增强宿主免疫功能的抗肿瘤作用。本研究拟从整体、离体、细胞水平，应用Dectin-1抑制剂系统研究Dectin-1对黑灵芝多糖免疫调节和抗肿瘤作用的影响；进一步综合应用现代分子生物学技术研究Dectin-1的黑灵芝多糖抗肿瘤作用的免疫机理，深入探讨黑灵芝多糖抗肿瘤作用机制。本研究可为肿瘤营养干预与防治及对黑灵芝的深入开发应用提供理论依据，也可为类似结构食源性组分研究拓展思路。

中文关键词： 黑灵芝多糖；功能食品；β-葡聚糖受体Dectin-1；抗肿瘤作用；免疫机理

英文摘要： Polysaccharide research is becoming one of the hotspots and difficulties in current life science research. Ganoderma atrum polysaccharide, a highly purified, β-glucan isolated from Ganoderma atrum, has been reported to exert anti-tumor effect via the enhancement of host immunity. However, little is known about the potential mechanisms for the antitumor immune responses of Ganoderma atrum polysaccharide. A recent discovery, the β-glucan receptor Dectin-1 provides new insights into the immune responses of β-glucans. And the characterization of Dectin-1, in particular, plays a positive role in promoting the mechanisms underlying the anti-tumor effect of β-glucans. The aim of this study is to investigate whether β-glucan of Ganoderma atrum polysaccharide is recognized by Dectin-1, and further examine immune mechanisms of Ganoderma atrum polysaccharide-induced anti-tumor effect. In the present study, the Dectin-1 inhibitor is thus used to evaluate the effect of Dectin-1 on the immunomodulation and anti-tumor effect of Ganoderma atrum polysaccharide in the overall level, level of organs and cell level. Modern molecular biotechnologies are further used to investigate the immune mechanisms for the anti-tumor effect of Ganoderma atrum polysaccharide involved in the regulation of Dectin-1, elucidating the antitumor mechan

英文关键词： Ganoderma atrum polysaccharide；Function food；Dectin-1；Anti-tumor effect；Immune mechanism