糖尿病肾病中医体质易感性与TGF-β#22522;因T869C(Leu 10 Pro) 多态性的相关性研究

项目名称： 糖尿病肾病中医体质易感性与TGF-β#22522;因T869C(Leu 10 Pro) 多态性的相关性研究

项目编号： No.30801467

项目类型： 青年科学基金项目

立项/批准年度： 2009

项目学科： 生物科学

项目作者： 牟新

作者单位： 浙江中医药大学

项目金额： 20万元

中文摘要： 本研究通过提出体质易感性—#35777;候多样性—#22522;因多态性假说，采用量表这一流行病学调查方法对360例2型糖尿病和糖尿病肾病患者进行横断面研究，以及2型糖尿病和糖尿病肾病患病人群TGF-β#22522;因T869C多态性。通过对DN患者的9种体质分型与TGF-β#22522;因T869C多态性的有关的因素采用二分类logistic回归分析，发现痰湿质、湿热质、瘀血质的DN患者和TGF-β#22522;因T869C多态现象有相关性。我们对DN患者的8种证侯与TGF-β#22522;因T869C多态性的有关的因素采用二分类logistic回归分析，发现最后无相关证侯进入回归模型。初步确定了该病的中医体质易感人群，不同体质糖尿病肾病患者在发生发展过程中出现不同的中医证候，阐明中医体质的部分分子生物学基础。建立具有创新性的糖尿病肾病中医病因学理论，促进中医学术进步，并为糖尿病肾病早期干预的可行性，为辨体质、辨病、辨证“#19977;位一体”#30340;糖尿病肾病辨证模式的建立奠定坚实的研究基础。

中文关键词： 2型糖尿病；糖尿病肾病；转化生长因子β#65307;中医体质；多态现象(遗传学)

英文摘要： To explore the association of Chinese medcine constitution susceptibility to diabetic nephropathy (DN) and TGF-β (T869C) gene polymorphism. TGF-βgene polymorphism was detected with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and screened for 180 DN cases. Patients with DN were surveyed epidemiologically with constitution in Chinese medicine questionnaire (CCMQ). Binary logistic regression analysis was utilized to study the correlation. All patients had similar base line characteristics. Nine types of Chinese medicine constitution and TGF-β(T869C) gene polymorphism were analyzed in patients with DN. And we found that the phlegm-dampness constitution, damp-heat constitution, blood stasis constitution have correlation with TGF-β (T869C) gene polymorphism. Different Chinese medicine constitutions were associated with TGF-β(T869C) gene polymorphism in DN patients.Differently constitutional of TCM in DN patients will appear differently syndrome of TCM,and clarify the basic of Biochemistry and Molecular Biology in constitutional and syndrome of TCM.In order to establish the theory of TCM etiology in DN and interfere this disease in earlier period through the research,and try to establish the differentiation of syndrome,disease and constitutional of TCM mode.

英文关键词： type 2 diabetes;diabetic nephropathy; TGF-β Chinese medicine constitution;gene polymorphism