多模态分子影像活体示踪神经干细胞联合内皮祖细胞移植治疗缺血性脑卒中的实验研究

项目名称： 多模态分子影像活体示踪神经干细胞联合内皮祖细胞移植治疗缺血性脑卒中的实验研究

项目编号： No.81501522

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 李沛城

作者单位： 苏州大学

项目金额： 18万元

中文摘要： 神经干细胞（Neural stem cells, NSCs）联合内皮祖细胞（Endothelial progenitor cells, EPCs）移植是一种非常有前景的治疗缺血性脑卒中的新方法，然而如何检测和评估其在活体中的迁徙归巢、增殖分化是限制其在临床应用的重要瓶颈。目前传统的观察方法难以实现在活体上对干细胞的迁徙和分化进行动态观测。本项目拟用带有荧光素酶-增强型绿色荧光蛋白的慢病毒载体和超顺磁性氧化铁-近红外分子影像探针分别标记EPCs和NSCs，然后将其经静脉联合移植至小鼠缺血性脑卒中模型体内，运用生物发光、磁共振和近红外多模态分子成像技术，活体动态示踪NSCs及EPCs的时空分布、增殖分化、相互作用、转归等生物学行为。本研究将为NSCs联合EPCs移植后的活体动态示踪提供一个新的手段，也为NSCs联合EPCs移植治疗缺血性脑卒中的临床应用提供实验基础。

中文关键词： 分子影像探针；缺血性脑卒中；干细胞移植；光学成像；磁共振分子成像

英文摘要： Neural stem cells (NSCs) joint endothelial progenitor cells (EPCs) transplantation has been a very promising treatment for ischemic stroke, however, how to evaluate the in vivo mobilization and homing, proliferation and differentiation is the important bottleneck of restricting its clinical application. At present, the traditional method is difficult to observe dynamically the migration and differentiation of stem cells in vivo. In this project we use luciferase-enhanced green fluorescent protein virus vector and superparamagnetic iron oxide and near-infrared probe to label EPCs and NSCs, respectively, and then transplant cells into mouse model to treatment ischemic stroke. We use the in vivo bioluminescence, magnetic resonance and near-infrared multi-modality imaging techniques to monitor the space-time distribution, proliferation, differentiation, and biological behavior of NSCs and EPCs after transplantation. The successful completion of this project will provide new means to trace the stem cells after transplantation, and lay out solid base for their potential clinical application.

英文关键词： Molecular imaging probes;Ischemic stroke;Stem cell transplantation;Optical imaging;Magnetic resonance molecular imaging