染色质重构蛋白CHR5在拟南芥抗病免疫反应中的功能研究

项目名称： 染色质重构蛋白CHR5在拟南芥抗病免疫反应中的功能研究

项目编号： No.31500215

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 邹保红

作者单位： 南京农业大学

项目金额： 20万元

中文摘要： 染色质重塑是通过表观遗传修饰调控基因表达的重要方式。染色质重构蛋白依靠水解ATP 释放的能量改变染色质结构，是染色质重塑的主要调控者之一。拟南芥中有42个sucrose non-fermenting two（SNF2）家族的染色质重构蛋白，它们中大部分蛋白的功能未被研究。本项目前期通过对拟南芥自体免疫（autoimmunity）突变体bon1-1表型抑制突变体的遗传筛选，发现染色质重构蛋白CHR5与抗病免疫反应相关。基于此我们拟通过：1）遗传筛选CHR5参与拟南芥抗病免疫反应调控的互作基因；2）转录组高通量测序（RNA-Seq）和染色质免疫共沉淀（ChIP）鉴定CHR5参与抗病免疫反应调控的靶标基因；3）酵母双杂交和双分子荧光互补（BiFC）鉴定CHR5参与抗病免疫反应调控的互作蛋白等研究，进一步解析CHR5在拟南芥抗病免疫反应中的功能，为其它植物染色质重构蛋白功能研究提供理论依据。

中文关键词： 植物抗病性调控；防卫反应；自体免疫突变体；分子机制；拟南芥

英文摘要： Chromatin remodeling is one important epigenetic modification that regulates gene expression by changing the structure of the chromatin. Chromatin remodeling ATPase protein, a major player in the process, utilizes energy derived from ATP hydrolysis to alter histone-DNA contacts within the nucleosome. There are 42 putative SNF2 ATPases in Arabidopsis, many of which have un-known functions. Recently, chromatin remodeling ATPase protein 5 (CHR5) was identified as a regulator of the plant immunity by the genetic suppressor screening of the autoimmunity mutant bon1-1. This study aims at further elucidating the regulatory mechanism of CHR5 in Arabidopsis immunity. Specific objectives are: 1) isolation of genetic interactor of CHR5 in Arabidopsis immunity by suppressor screen; 2) identification of direct regulatory targets of CHR5 by ChIP (chromatin immuno-precipitation) in Arabidopsis immunity; 3) identification of CHR5 interaction proteins by yeast two hybrid screen and bimolecular fluorescence complementation. The above study will elucidate the regulatory mechanisms of CHR5 in Arabidopsis immunity and provide a theoretical basis for the function study of other plant chromatin remodeling ATPase proteins.

英文关键词： plant disease resistance regulation;defense response;autoimmunity mutant;melecular mechanism;arabidopsis