项目名称: 基于大片段基因组DNA植物表达文库的复苏植物耐脱水机理研究
项目编号: No.31270312
项目类型: 面上项目
立项/批准年度: 2013
项目学科: 生物科学
项目作者: 邓馨
作者单位: 中国科学院植物研究所
项目金额: 88万元
中文摘要: 复苏植物生活于极端干旱生境,具有极强的耐脱水能力,失水95%以上仍然干而不死,其特殊的抗旱机制值得关注。以往研究主要针对干旱诱导基因,忽视了那些组成型表达的基因,和基因簇、染色质修饰、基因互作、非编码序列等基因组水平上的调控对抗旱性状的影响。这主要是因为复苏植物的遗传信息不清楚、遗传研究的体系不成熟,常规"组学"研究难以深入。本项目在多学科交叉研究的基础上借鉴遗传学策略提出新思路,利用可以携带大片段DNA的BIBAC植物表达体系将复苏植物牛耳草的大片段基因组DNA文库直接转入拟南芥创建转化群体,通过对转化群体中转基因株系进行系统的抗旱性表型比较来鉴定具有提高植物抗旱性功能的大片段DNA,进一步克隆其所包含的关键基因与调控序列,最终在基因组层面上高通量地鉴定旋蒴苣苔的抗旱基因/基因簇/非编码序列,分析其中可能存在的基因互作、修饰和调控,从而深化植物抗旱分子调控理论,为抗旱遗传育种提供基础。
中文关键词: BIBAC文库;复苏植物;抗旱性;小RNA;转座子
英文摘要: Resurrection plants inhabit in extream drought habitat and exhibit desiccation tolerance as it can survive after 95% water lost,thus represent a good model for studies on severe drought resistance.Most studies have focused on the dehydration-induced genes and neglected those constitutively expressed genes, and gene clusters, gene interactions and chromosomal modifications and noncoding sequences on the genome. The main reason is that the lack of genetic information on the genome of resurrection plants and effective genetic manipulation system limits the use of "-omics" approaches.Recently, we have construced a library of high-molecular weight(MW) genomic DNA from Boea hygrometrica, a typical resurrection plant distribute widely in China, using a binary vector of BIBAC series that can be directly used to transform Agrobacteria and integrate the inserted large DNA frament into plant genome. In this project,this library will be used to construct a transgenic population of Arabidopsis haboring high-MW genomic DNA fragments of Boea hygrometrica. The exogenous high-MW DNA fragments that are functional to improve transgenic plant drought resistance will be picked out by comparing the sensitivity to dehydration stress. Furthermore,the genes and noncoding sequences in the candidate large DNA fragments and the possible in
英文关键词: BIBAC library;resurrection plant;desiccation tolerance;small RNA;retro-transposon