CD36调控多囊卵巢综合征女性脂代谢异常的分子机制研究

项目名称： CD36调控多囊卵巢综合征女性脂代谢异常的分子机制研究

项目编号： No.81471427

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 李蓉

作者单位： 北京大学

项目金额： 74万元

中文摘要： 多囊卵巢综合征（PCOS）患者在我国育龄女性人群中发病率达到5.6%，同时此类患者脂代谢异常发生率高，使其远期并发症高血压、糖尿病、心血管疾病发病率明显高于非PCOS育龄女性人群。CD36在脂类物质代谢中处于信号通路的上游，其表达异常与PCOS患者脂代谢异常密切相关，但是其作用的分子机制还不清楚。本研究拟通过高通量组学技术，研究PCOS患者肌肉细胞的转录情况，明确与脂代谢相关的差异表达基因，分析与CD36表达有相关性的调控通路，并通过动物研究，明确调控CD36表达的新转录因子的功能，并预测可能与其相关的细胞因子，进而利用细胞学与动物学实验，验证预测准确性的同时，探索是否可以通过细胞因子，达到治疗PCOS异常脂代谢病理现象的目的。我们的研究为明确PCOS病理提供了理论借鉴，同时对于寻找预防和治疗PCOS脂代谢异常的药物提供了理论指导。

中文关键词： 多囊卵巢综合征；生殖内分泌；脂肪代谢；基因表达；肌肉

英文摘要： It is known that the prevalence of polycystic ovarian syndrome (PCOS) has reached to 5.6 percent in Chinese community women at reproductive ages. Moreover abnormal lipid metabolism has been observed in large part of women with PCOS, which resulted in the increased incident of complications including hypertension, diabetes and cardiovascular diseases. CD36 is an upstream molecular in the signal pathway of regulating lipid metabolism, and the aberrated expression profiling of CD36 has been proved the positive role in the PCOS women with impaired lipid metabolism, however it is still unclear for its molecular mechanism in the process. In the present study, genomic transcription is studied by gene chips using muscle cells from PCOS women, and the genes related to lipid metabolism is screened and the signal pathway that regulated CD36 activity is identified. Differentiated metabolism products will be predicted by metabonomics and gene expression, and their function will be tested by cellular and animal model study to improve the phenotype of PCOS. Our project will provide the theoretical direction of PCOS etiology, and facilitate to further study of drug discovery for prevention and treatment of abnormal lipid metabolism in PCOS women.

英文关键词： polycystic ovarian syndrome;reproductive endocrinology;lipid metabolism;gene expression;muscle