基于“双功能”因子调控d-PET过程的近红外比率荧光探针的构建及重金属离子识别性能研究

项目名称： 基于“双功能”因子调控d-PET过程的近红外比率荧光探针的构建及重金属离子识别性能研究

项目编号： No.U1304202

项目类型： 联合基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 许志红

作者单位： 许昌学院

项目金额： 31万元

中文摘要： 近红外比率荧光探针能有效降低生物样品自吸收和自发荧光、光散射、探针浓度及检测环境等因素的干扰，并能深入到组织内部，检测灵敏度高且更适合荧光成像，在重金属离子荧光探针设计中受到广泛关注。本项目拟选取七甲川花菁染料为近红外荧光团，罗丹明衍生物为“双功能”调控因子，用具有重金属离子识别性能的连接基把二者联接起来；通过重金属离子诱导下调控因子“闭-开”环结构转变后供吸电子能力的逆转，调节花菁染料激发态到调控因子的d-PET(donor-excited PET)过程，同时调控因子又作为新的荧光信号输出单元；从而构建大发射光谱位移的高分辨近红外比率荧光探针，并应用于重金属离子的荧光成像及环境水样分析。该项目的成功实施可为新颖近红外比率荧光探针设计策略的提出以及合成性能更加优异的重金属离子荧光探针提供重要理论依据和数据支持，同时也可使基于单一罗丹明或花菁染料的传统荧光探针设计焕发新的生机。

中文关键词： 双功能调控因子；供体激发态光诱导电子转移；近红外；比率荧光探针；小分子

英文摘要： Most attention has been paid to near-infrared(NIR) ratiometric fluorescent probes in the design of heavy metal ions fluorescent probes, causing the NIR ratiometric probes exhibits special advantages including minimal interfering absorption and fluorescence from biological samples, low scattering, and deep penetration into tissues, and eliminating disadvantage of the excitation backscattering, the probe concentration and environmental conditions effects, which have high sensitivity and is more suitable for cell imaging. In this program, the heptamethine cyanine was selected as a NIR fluorophore, and rhodamine derivatives was chosen as a dual-function modulator to construct a whole system connected by linker which have recognition character. We speculated that modifying an electron-donating rhodamine spirolactam group into a heptamethine dye platform that could be specially induced by metal ions to convert spriolactam form to an electron-drawing opening form will activate the d-PET process of cyanine to rhodamine and emit another fluorescence simultaneously, provide a shift switch for ratiometric detection of metal ions and apply to living cell imaging and analysis of water samples. This program can supply important data and theory acknowledge for exploring novel designing strategy of NIR ratiometric probes and sy

英文关键词： Dual-function modulator；d-PET；Near-infrared；Ratiometric fluorescent probes；Small molecules