新型双效细胞内自由基清除剂的三维结构及其放射防护效应的选择机制

项目名称： 新型双效细胞内自由基清除剂的三维结构及其放射防护效应的选择机制

项目编号： No.81472907

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 潘剑茹

作者单位： 福州大学

项目金额： 70万元

中文摘要： 肿瘤放疗副作用的防治是影响放射治疗疗程能否正常实施以及能否完成的关键，阿米福汀是目前唯一临床使用的放射防护剂，但它有各种各样的副作用，而且无法穿越血脑屏障，在临床上的使用量越来越少，市场上急需一种新的安全高效的放射防护剂以替代阿米福汀。本项目研究的双效细胞内自由基清除剂GST-TAT-SOD已证实可通过常规给药方式进入细胞，还可穿越血脑屏障，选择性保护正常细胞免受放疗损伤的毒害。本项目拟系统研究双效细胞内自由基清除剂GST-TAT-SOD蛋白的三维结构及其抗放射损伤效应，从分子、细胞、组织乃至整体动物水平上阐明其放射防护的选择机制以及结构与功能的关系。本项目的成功研究不仅可为放射防护提供一个新的思路，丰富放射防护学理论，还可为新型低成本、高产量、无毒、高效的放疗防护剂奠定应用基础，解决肿瘤患者放疗副损伤的防治难题。

中文关键词： 放射防护；细胞内自由基清除剂；三维结构；选择性

英文摘要： Control the side effects of radiotherapy is not only the most critical influence on the complement of radiotherapy, but also one of the important reasons for the failure of cancer treatment. Amifostine is the only agent in current clinical radiological protection , but it has various side effects , and can not cross the blood-brain barrier. A novel effective and secure substitute is in a great need for the current market with the diminishing of clinical usage of amifostine due to those imperfection. Previous studies have shown that bifunctional eliminator of the intracellular free radicals GST-TAT-SOD has the ability of entering cells, even acrossing the blood-brain barrier ,in various regular drug-delivery way and selectively protect normal cells against radiation damage. On these basis, this study will research this research will develop for the three-dimensional structure and radioprotective effect of GST-TAT-SOD in order to illustrates the selective radioprotective mechanisms of the protein at the molecular, cellular, tissue, organ and whole animal level and the relations between its structure and function.The result will not only provide a new ideas for radiological protection, enrich the of radioprotective theory, but also establish the basis for the application of the novel low-cost, high-yield , non-toxic and efficient radiation protectant to overcome the prevention problems of radiotherapy side injury in cancer patients.

英文关键词： radioprotective;eliminator of the intracellular free radicals;three-dimensional structure;selective