益气活血法对脑出血后血管新生-胶质细胞活化相互作用的调节机制研究

项目名称： 益气活血法对脑出血后血管新生-胶质细胞活化相互作用的调节机制研究

项目编号： No.81202625

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 周华军

作者单位： 三峡大学

项目金额： 23万元

中文摘要： 血管新生与星形胶质细胞（AST）活化密切有关。AST活化通过分泌HIF-1α而促进血管新生过程，若AST过度活化形成胶质瘢痕，则对新生血管起屏障作用；血管内皮细胞通过LIF反馈抑制AST活化，因此HIF-1α/LIF是血管新生-AST活化相互作用的重要枢纽之一。我们前期工作证实益气活血法能促进脑出血后的血管新生，国内同行研究显示益气活血法具有调节脑缺血后星形胶质细胞活化，抑制胶质瘢痕形成的作用。推测益气活血法可能通过HIF-1α/LIF而调节血管新生与AST活化相互作用而优化脑出血后微血管重建过程。本项目拟从血管新生与AST活化相互作用的角度研究益气活血法对脑出血后血管新生、AST活化、HIF-1α/LIF时空表达的影响及其因果关系，进一步揭示益气活血法促进脑出血损伤组织修复的机理，为其临床上促进神经功能恢复提供更充分的实验依据。

中文关键词： 脑出血；血管新生；胶质细胞；益气活血法；

英文摘要： Angiogenesis and astrocyte (AST) activation are closely related. AST activation can promote angiogenesis by secreting HIF-1α, but if the AST were over activated and glial scar formation, which will be a barrier for angiogenesis. Leukemia inhibitory factor which is secreted from endothelial cells has been shown to inhibit AST activation as a negative feedback signal. So HIF-1α and LIF are the most important factors for the interaction between angiogenesis and AST activation. Our previous work has confirmed that Qi-tonifying and stasis-eliminating therapy can promote angiogenesis after intracerebral hemorrhage (ICH), and domestic researchers shows Qi-tonifying and stasis-eliminating therapy play a vital role in regulating astrocytes activation and inhibiting glial scar formation after ischemia as well. Hence, Qi-tonifying and stasis-eliminating therapy is supposed to regulate the interaction between angiogenesis and AST activation by HIF-1α and LIF, and optimize microvascular reconstruction after ICH. The purpose of this project is to establish the effect of Qi-tonifying and stasis-eliminating therapy on angiogenesis, AST activation, spatial and temporal expression of HIF-1α and LIF, and mutual causality after ICH, to further elucidate its mechanisms in reconstruction of microvascular networks, and to provide mo

英文关键词： intracerebral hemorrhage；angiogenesis；glia cell；Qi-tonifying and Stasis-eliminating；