基于超分子自组装体制备荧光Au纳米点及基因转染应用研究

项目名称： 基于超分子自组装体制备荧光Au纳米点及基因转染应用研究

项目编号： No.U1204213

项目类型： 联合基金项目

立项/批准年度： 2013

项目学科： 高分子科学

项目作者： 石云峰

作者单位： 安阳师范学院

项目金额： 30万元

中文摘要： 具有荧光性能的Au纳米点在自组装、相转移、生物医学等领域具有广泛的应用研究。目前，Au纳米点多是采用常规的共价型树形聚合物制备，尚未有采用基于树形聚合物构筑的超分子自组装体制备Au纳米点的报道。基于超支化聚合物构筑的超分子自组装体拥有超分子聚合物的动态可逆特性，又具有三维准球形高度支化的结构，是一类非常理想的制备智能Au纳米点的载体。通过树形聚合物构筑的超分子组装体制备Au纳米点，结合了智能聚合物的环境敏感特性、生物相容性与Au纳米点的光、电等特性，能够赋予荧光Au纳米点以智能特性和生物相容性。本项目拟基于超支化聚胺和脂肪酸构筑超分子自组装体，并用于制备具有荧光和智能特性的Au纳米点，研究Au纳米点在水油两相的可控转移，探索Au纳米点/超分子自组装体在基因转染中的应用。

中文关键词： 超支化聚合物；超分子自组装体；金属纳米点；量子点；基因转染

英文摘要： Fluorescent Au nanodots have been extensively investigated on self-assembly, phase transfer, bio-medicine, etc. Until now, Au nanodots are mostly prepared in the presence of covalent dendritic polymers and there has no report on synthesizing Au nanodots within supramolecular self-assembly which is constructed by dendritic polymers. The supramolecular self-assembly based on dendritic polymers not only have the dynamic reversible properties of supramolecules, but also possess torispherical and highly branched architectures. Thus they can be regarded as a new kind of ideal nanoreactors for preparing intelligent Au nanodots. By preparing Au nanodots within supramolecular self-assembly based on dendritic polymers as nanoreactors, the environment sensitivity and biocompatibility of intelligent polymers and the optical, electrical properties of Au nanodots can be combined, endowing the nanodots with intelligence and biocompatibility. In this program, we would like to design supramolecular self-assembly based on dendritic polymers and then to prepare high fluorescent and intelligent Au nanodots. The phase transfer of Au nanodots between water and oil phase and gene transfection application of the resulting Au nanodots/supramolecular self-assembly nanocomposites would be also investigated.

英文关键词： hyperbranched polymer；supramolecular self-assembly；metal nanodots；quantum dots；gene transfection