催化释放一氧化氮的手性微环境及其细胞相容性研究

项目名称： 催化释放一氧化氮的手性微环境及其细胞相容性研究

项目编号： No.31270020

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 翁亚军

作者单位： 西南交通大学

项目金额： 80万元

中文摘要： 手性是生物系统所具有的独特性质，手性识别增加了复杂生物系统中相互作用的选择性，是各种生物学功能得以正确实现的关键。在申请者前期仿生催化活性构建及其原位诱导一氧化氮（NO）释放的研究基础上，本项目拟在催化活性表面进一步引入手性构象，使材料表面与蛋白、细胞之间具有手性识别或选择性相互作用，进一步改善材料表面的生物相容性，获得兼顾内皮细胞相容的释放一氧化氮的催化活性手性表面。通过复杂体系中典型血浆蛋白在手性表面的吸附与催化释放NO的评价；体外细胞相容性分析、血液相容性分析、动物体内植入实验等系统性研究，获得手性微环境与蛋白、血液、细胞作用的规律以及对催化活性的影响。由于生物材料与生物界面选择性相互作用的重要性，本项目的研究将为性能优异的生物材料的设计与制备提供必要的科学依据。

中文关键词： 手性；催化活性；一氧化氮；内皮细胞；血液相容性

英文摘要： Chirality is the unique nature of biosystems, and chiral recognition increases the selective interaction which is the key factor of correct realization of multiple biofunctions in complex biosystems. Based on the preliminary study of constructing of catalytically bioactive surface to induce nitric oxide (NO) release, this project will introduce chiral conformation on the catalytically bioactive surface thus to introduce chiral recognition or selective interaction between materials surface and proteins or cells. The objects are to further improve of the biocompatibility and to obtain a combined property of endothelial compatibility and catalytic NO release. By evaluation of adsorption of typical blood proteins on the chiral surface and catalytic NO release in complex systems, and also by cytocompatibility analysis, blood compatibility analysis and in vivo implantation research, the law of interaction between and proteins, blood or cells as well as the influence of chiral microenvironment on catalytic activity will be explored. Since it is very important of the selective interaction between biomaterials and biointerfaces, this study will provide essential scientific basis for designing and developing outstanding performance biomaterials.

英文关键词： chirality；catalytic activity；nitric oxide；endothelial cells；blood compatibility