针刺调控VD大鼠海马星形胶质细胞活化增殖作用及其NLRC5-NF-κB信号机制研究

项目名称： 针刺调控VD大鼠海马星形胶质细胞活化增殖作用及其NLRC5-NF-κB信号机制研究

项目编号： No.81202739

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 张雪竹

作者单位： 天津中医药大学

项目金额： 23万元

中文摘要： 以炎症因子升高为特点的慢性胶质细胞活化，是血管性痴呆（VD）的重要病理特征。脑缺血后星形胶质细胞（AC）首先受损并活化增殖，分泌大量炎症因子，导致神经元死亡；本身还形成胶质瘢痕，阻碍轴突生长及神经功能修复。 NF-κB是AC炎症反应的核心及多种炎症因子诱导AC活化增殖的最后效应通道；NLRC5负调控NF-κB通路活性，抑制其介导的炎症反应。说明NLRC5-NF-κB-AC活化增殖间可能存在一定联系。我们预实验发现，针刺能抑制VD鼠海马AC活化增殖，减少神经元凋亡；良性调节NLRC5和NF-κB表达。证实上述联系客观存在，也提示针刺调节AC活化增殖的作用与NLRC5水平密切相关。 因此本项目拟将NLRC5与AC活化增殖相联系，基于炎症反应这一相通环节，采用流式细胞术、免疫细胞化学、分子生物学技术，研究NLRC5在AC活化增殖中的作用及针刺影响。为揭示针刺治疗VD的作用机理提供实验依据。

中文关键词： 血管性痴呆；星形胶质细胞；细胞周期；核因子-κB；NLRC5

英文摘要： Ischemic cerebrovascular disease is the major risk factor for vascular dementia (VD). After ischemic injuries, astrocytes (AC) were firstly damaged, rapidly activated and proliferating, secrete large amounts of inflammatory factors and lead to neuronal death; astrocytes reactive gliosis, formate glial barrier, inhibit axonal growth, repair Neural circuits, and influence the reconstruction of nerve function. NF-κB is the core of the AC inflammation and the final effect channel of a variety of inflammatory factors inducing AC activation and proliferation, and play a key role in the development of VD neurodegenerative. NLRC5 is a key negative regulator of NF-κB pathway and can inhibit NF-κB-mediated inflammatory response. Preliminary studies found that acupuncture could reduce the neuronal apoptosis of VD rat hippocampal, inhibit AC activation and proliferation, and regulate NLRC5 and NF-κB expression. The current study intends to link NLRC5 and AC activation and proliferation, and observe the relationship and working mechanism between NLRC5 and VD rats hippocampal AC activation and proliferation, through flow cytometry, immunocytochemistry, molecular biology techniques. And the study was based on the same inflammatory response and the research platform of acupuncture effect. The current study intends to clarify th

英文关键词： Vascular Dementia；astrocyte；cell cycle；NF-кB；NLRC5