电活性微生物聚集体中胞外电子传递网络的分子构成与运行机制

项目名称： 电活性微生物聚集体中胞外电子传递网络的分子构成与运行机制

项目编号： No.21507123

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 化学工业

项目作者： 李道波

作者单位： 中国科学技术大学

项目金额： 21万元

中文摘要： 微生物胞外电子传递是近年来环境微生物学领域的重要发现，它的作用机制关系到多种元素的地球化学循环和新型生物能源技术的开发。在群落水平上对微生物远程电子传递网络进行解析是当前深入理解微生物电化学活动基本原理的迫切任务。申请人拟以电活性微生物聚集体中胞外电子传递网络为核心，充分借助分析化学、（光谱）电化学和分子生物学的先进技术手段，综合描述聚集体胞外电子传递网络的空间结构、分子组成和电活性，并且从聚集体内生物代谢活性的角度观测远程电子传递的实际功能和作用机制，解析远程电子传递区别于细胞表面直接放电的本质机理，并力图建立基于实验观测结果的微生物远程电荷传导模型。

中文关键词： 微生物燃料电池；微生物聚集体；细胞色素c；电子传递；氧化还原媒介

英文摘要： Extracellular electron transfer of microorganisms is an important discovery in recent years in the field of environmental microbiology. Its performance is closely related to the geochemical cycles of multiple elements and the development of new bio-energy apparatuses. Parsing the remote electron transfer network on the level of microbial community is currently urgent for in-depth understanding of the principles of microbial electrochemistry. The applicant intends take the extracellular electron transfer network in the electroactive microbial aggregates as the core for study, making full use of advanced technologies in analytical chemistry, (spectro)electrochemistry, and molecular biology. Comprehensive descriptions on the spatial topology, molecular structure, and electrical activity of the electron transport network in the extracellular aggregates will be conducted. The biological function and action mechanism of remote electron transfer will be observed from the perspective of metabolic activity. The applicant will also analyze the essential difference between remote electron transfer and direct discharging on cell surface, and tried to establish remote conduction model for microbial aggregates based on the experimental results.

英文关键词： microbial fuel cell;microbial aggregate;cytochrome c;electron transfer;redox mediator