用于生物单分子检测的新型二维纳米材料的按需构筑方法及机理研究

项目名称： 用于生物单分子检测的新型二维纳米材料的按需构筑方法及机理研究

项目编号： No.51302037

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 一般工业技术

项目作者： 谢骁

作者单位： 东南大学

项目金额： 24万元

中文摘要： 以石墨烯、氮化硼、二硫化钼等为代表的新型二维纳米材料按需构筑的纳米孔器件及其检测技术被认为是最具发展潜力的生物单分子检测技术，特别是纳米孔基因测序技术，是实现个性化医疗的重要手段。国内外研究表明，二维纳米孔在加工时受到外场作用，特别是受电子辐照和热场而产生的结构演化是研究热点之一。此外，纳米材料实现微观可控的转移并按需构筑纳米结构迄今尚十分困难。本课题将发展辐照诱导的分子差异化修饰和二维纳米材料的自组装按需构筑方法，运用原位电子显微技术研究二维纳米材料在电子束加工和热场作用下的结构演化规律。通过生物实验，证实用于生物单分子检测的按需构筑纳米孔结构的可靠性。课题将致力于解决纳米孔生物检测中核心材料及制备问题，并解释加工过程中外场作用影响及物理机制。

中文关键词： 二维材料；石墨烯；基因组测序；纳米孔；电子显微学

英文摘要： On-demand construction of nanopore device based on novel two-dimensional nanomaterials, e.g. graphene, boron nitride and molybdenum disulfide, is believed the most practical single biomolecular identification technique nowadays. Specifically, nanopore gene sequencing technique is believed an important route to personalized medicine. Global researches indicate that the structural evolution of 2-D nanopores under external excitation when being processed, especially under electron irradiation and thermal field is a leading hotspot in the world. However, up till now, on-demand construction of nanostructures through microscopically controllable transfer of nanomaterials still shows difficuties. This proposal aims to delelop irradiation-mediated, differential molecular modification and on-demand self-assembly strategy of 2-D nanodevices. In-situ electron microscopy will be applied to study structural evolution of 2-D nanomaterials under electron irradiation and thermal field. We attempt to prove the reliability of on-demand construction of nanopore structures by biological assays. The goal of this proposal is to solve the basis of materials and device fabrication process in nanopore biosensing, as well as to point out the effect of external field during fabrication process and the corresponding mechanisms.

英文关键词： 2D materials；graphene；genome sequencing；nanopore；electron microscopy