miRNA调控细胞自噬及其在PRRSV增殖中作用与机制研究

项目名称： miRNA调控细胞自噬及其在PRRSV增殖中作用与机制研究

项目编号： No.31272427

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 张淑君

作者单位： 华中农业大学

项目金额： 80万元

中文摘要： 猪繁殖与呼吸系统综合症（PRRS）是危害大控制难的世界性传染病，目前尚无有效的防治药物和技术。研究表明miRNA不仅对细胞自噬及病毒增殖等具有重要作用，还是药物设计的有效新型靶分子。我们前期研究发现PRRS病毒能导致miRNA差异表达和细胞自噬，miRNA和自噬均参与调控PRRSV增殖、且两者间存在互作；然而miRNA调控自噬及其在PRRSV增殖中作用与机制尚不清楚。本项目将研究miRNA对自噬和PRRSV增殖调控效应，探讨miRNA对自噬相关信号通路和重要基因的作用及其对PRRSV增殖的调控网络与机理，旨在：①阐明自噬在PRRSV增殖中作用及其机理；②解析miRNA对感染PRRSV后细胞自噬的调控作用与机理；③挖掘参与调控自噬及PRRSV增殖的关键基因、miRNA及其靶基因，为阐明宿主与PRRSV互作、宿主抗病机制奠定基础，为防治PRRS新药设计的靶分子和抗病新品种的培育提供理论依据。

中文关键词： 猪繁殖与呼吸系统综合症病毒；细胞自噬；线粒体自噬；病毒复制；小RNA

英文摘要： Porcine reproductive and respiratory syndrome (PRRS) is a viral disease with huge economic losses that is difficult to control in the word, and there are no effective medicines and techniques for prevention and treatment at present. The literatures reported that miRNA is an important regulator in cell autophagy and virus proliferation, and also the new effective target molecule for medicine design. In our previous studies, we identified some differently expressed-miRNA and cell autophagy were triggered by PRRSV infection. In addition, we also found that these miRNA and cell autophagy not only regulated the PRRSV proliferation, but also there were interaction between them. However, the regulatory effect and mechanism of these miRNA on the cell autophagy and PRRSV proliferation are not clear. In this study, the regulatory effect of these above miRNA on cell autophagy and PRRSV proliferation will be investigated, and the regulatory effect of miRNA on signaling pathways of cell autophagy and the important genes on the pathways, and the regulation network and mechanism of PRRSV proliferation will also be explored. The aims of this study are to explain the regulatory effect and mechanism of miRNA on cell autophagy infected by PPRSV, and of cell autophagy on PRRSV proliferation, and also to discover the key genes, miRN

英文关键词： PRRSV；autophagy；mitophagy；Virus replication；miRNA