从母系遗传及端粒衰老学说研究肾虚在慢性再障中的发病机制

项目名称： 从母系遗传及端粒衰老学说研究肾虚在慢性再障中的发病机制

项目编号： No.81202839

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 崔兴

作者单位： 山东中医药大学

项目金额： 23万元

中文摘要： 慢性再生障碍性贫血中医病因病机多责之于"肾虚"，依据中医传统理论"肾为先天之本"、"肾主藏精"、"肾主骨生髓"，"肾精"与骨髓细胞增殖、分化、衰老乃至凋亡等基本生命活动相关。第一部分研究由《内经》中"以母为基"联系到了先天之"阴精"，并结合线粒体"母系遗传"、"能量工厂"的特性，得出了中医的"阴精"即是母系遗传的根本- - "线粒体"的论点。研究表明，端粒衰老学说在慢性再障骨髓衰竭过程中起到了至关重要的作用，这与中医的肾虚衰老学说遥相呼应。基于以上两点，设计了该课题。 课题组既往对慢性再障线粒体的研究发现，肾阴虚型患者发现许多突变位点。本课题拟通过收集肾阴虚、肾阳虚、肾阴阳两虚慢性再障患者骨髓，检测线粒体突变、电镜观察线粒体形态；检测端粒长度、端粒酶的活性；研究其与慢性再障中医辨证分型的相关性，以期寻找中医宏观理论与现代医学微观指标的结合点，并为中医药治疗慢性再障找到分子生物学水平的依据。

中文关键词： 线粒体；端粒；肾虚；再生障碍性贫血；代谢组学

英文摘要： The etiology and pathogenesis of chronic aplastic anemia is attributed to the deficiency of kidney.From the traditional Chinese medicine theory of "kidney is the congenital foundation", "Lord of renal hidden essence" and "kidney dominating bone marrow" ,we can found that "kidney essence"are closely related to the proliferation, differentiation, senescence and apoptosis of bone marrow cells. On the first part ,we sought to link the " Life comes from essence of parents " of "Huang Di Nei Jing" with the "Yin essence" of cingenital, and with the properties of mitochondrial DNA-the maternal genetic, we draw a conclusion that the "Yin essence" is the maternally inherited fundamental - - "mitochondria" .Recent studies have revealed, telomere aging theory in aplastic anemia bone marrow failure process played a crucial role, this is square with the notion of traditional Chinese medicine kidney aging theory . Based on the above two points, we design the topic. We found that there are many mutations of mitochondrial DNA in kidney yin deficiency group patients with chronic aplastic anemia.Therefore, in this paper,we are trying to detect the mitochondrial DNA mutations; the telomere length and the telomerase activity,to find the relations of mitochondrial mutations, telomere-telomerase and the TCM Syndrome Types, in order

英文关键词： mitochondria；telomere；kidney deficiency；aplastic anemia；Metabolomic