The most realistic information about the transparent sample such as a live cell can be obtained only using bright-field light microscopy. At high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response from an observed sample using a bright-field microscope equipped with a high-resolution (4872x3248) image sensor. In order to suppress data distortions originating from the light interactions with elements in the optical path, poor sensor reproduction (geometrical defects of the camera sensor and some peculiarities of sensor sensitivity), we propose a spectroscopic approach for the correction of this uncompressed 12-bpc data by simultaneous calibration of all parts of the experimental arrangement. Moreover, the final intensities of the corrected images are proportional to the photon fluxes detected by a camera sensor. It can be visualized in 8-bpc intensity depth after the Least Information Loss compression.
翻译:在高强度脉冲LED光化中,我们用一个装有高分辨率(4872x3248)图像传感器的亮度显微镜从观测的样品中采集到一个12位/每声道(bpc)的初级反应。为了抑制光度与光学路径元素的光度互动产生的数据扭曲、低传感器复制(相机传感器的几何缺陷和传感器敏感度的某些特殊性),我们提议一种光谱法,通过同时校准实验安排的所有部分来纠正这一未压缩的12位/每声道数据。此外,经过校正的图像的最后强度与摄像传感器检测到的光通量成比例。在最小信息损失压缩后,可以在8位/bc强度深度以8摄氏度的速度进行视觉分析。