与AEC共培养对AMSC在NOD/SCID小鼠体内归巢及造血支持作用的影响

项目名称： 与AEC共培养对AMSC在NOD/SCID小鼠体内归巢及造血支持作用的影响

项目编号： No.31260232

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 史明霞

作者单位： 昆明医科大学

项目金额： 50万元

中文摘要： 加快造血恢复对提高造血干细胞移植(HSCT)成功率、减少感染等移植并发症、改善患者生存质量具有重要意义。前期，我们发现增加骨髓间充质干细胞（BMSC）表面CXCR4的表达，可以有效促进MSC向骨髓归巢和加快受体造血恢复。但BMSC来源、数量、扩增和分化能力有限，使研究和应用受到限制。羊膜MSC(AMSC)来源丰富、增殖迅速、分离纯化方法简单，前期我们首次证实了AMSC体外具有与BMSC相似的支持造血作用，甚至表达比BMSC更丰富的造血细胞因子，并发现通过与羊膜上皮细胞（AEC）共培养可以有效增加AMSC表面CXCR4的表达，并保持良好细胞活性。本研究旨在观察AEC共培养对AMSC归巢能力的影响，探索应用AMSC加速造血恢复的可能性，阐明SDF-1/CXCR4轴在AMSC归巢、植入和促进造血过程中的作用。为AMSC临床应用奠定基础，并希望为临床提高MSC移植疗效和HSCT成功率提供新策略。

中文关键词： 羊膜；间充质干细胞；条件培养基；CXCR4；归巢

英文摘要： Although allogeneic hematopoietic stem cell (HSC) transplantation is an effective therapeutic modality for a variety of malignant and nonmalignant disorders, it remains complicated by treatment-related mortality because of infection and bleeding, regimen-related toxicity, engraftment failure, and acute and chronic graft-versus-host disease. Cotransplantation of mesenchymal stem cells (MSC) and HSC after myeloablative therapy could facilitate engraftment and accelerate hematopoietic recovery. And further opportunities have been identified to improve the clinical benefits after MSC transplantation via their homing/migratory capacity. Previously we have demonstrated the importance of the SDF-1/CXCR4 axis in the regulation of motility of Flk1+ MSC derived from human fetal BM. Increasing CXCR4 functional expression might be a potential strategy to improve engraftment of Flk1+ MSC in BM and accelerate hematopoiesis recovery.The human amniotic membrane is a highly abundant and readily available tissue. Amniotic MSC (AMSC) have been proposed as good candidates to be used in cell therapy or regenerative medicine to treat damaged or diseased tissues. And we have shown for the first time that the capacity of hematopoitic support of AMSC was similar to that of BMSC. Interestingly AMSC could even express the hematopoietic cy

英文关键词： amnion；mesenchymal stem cells；conditioned medium；CXCR4；homing