神经细胞放电动力学行为的全域光相位成像分析方法与技术

项目名称： 神经细胞放电动力学行为的全域光相位成像分析方法与技术

项目编号： No.11474134

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 季颖

作者单位： 江苏大学

项目金额： 93万元

中文摘要： 神经细胞放电动力学行为的研究在神经科学、信息科学研究和临床应用等领域有着重要的意义。传统动力学研究手段及电位检测技术在参数分析多维性及空间刻画连续性等方面尚显不足。本项目拟基于光相位理论及其显微成像技术，分析神经细胞物理结构和光学性质特征，建立其折射率和厚度表征下的光相位模型；分析电活动下多样性作用对折射率的影响，阐明作用机理，紧扣神经细胞膜电位-离子浓度关键物理量，探明其与细胞光折射率及成像厚度的关联，建立光相位特征量-电参量动态关系；提出全域细胞连续采样技术要求，研发匹配的全场瞬态相位成像装置，给出有效相位参量识别方法，提出可连续位点采样的相位快速提取、重建及计算方法；依据电特征-光相位联系的本质，构建光相位参量刻画下的动力学方程，给出求解和演化分析方法。从理论和实验两方面实现光相位表述下的神经细胞全域放电动力学行为瞬态分析，弥补传统方法的不足，为神经细胞放电活动分析提供一种新方法。

中文关键词： 光相位显微成像；神经细胞；动力学行为；电参量相位表征；连续位点采样

英文摘要： Dynamical analysis of the firing patterns of neurons is of great magnificance in many research fields, such as neuroscience, information science, clinical application and so on. Traditional techniques are still insufficient for mechanism analysis in multi-dimension as well as for potential measurement in continuity. This subject will study the firing behaviors of neurons in view of quantitative phase microscopy. Its main contents are presented as follows: The characteristics of physical structure and optical property of neurons will be investigated based on quantitative phase imaging in this subject. Phase models will be established according to the distribution of refractive index and thickness of the neurons. The exploration of the various effects of firing on the refractive index will be made to reveal the interaction between membrane potential (calcium ion concentration) and refractive index as well as thickness. Especially, the analysis will be made of the dynamic relationship between the characters of phase and the electrical parameters of the neurons. Technology requirements of continuous sampling for the universe of neurons will be given, and the full-field phase microscopic imaging device which matches this demand will be set up. Furthermore, the recognition methods of effective phase characteristic as well as the ultrafast extracted and retrieval process of the phase informations will be discussed. Finally, the dynamic equations expressed by phase characteristic and the corresponding analysis method will be proposed in this subject. This work will achieve the transient and full-field dynamical analysis of the firing patterns of neurons based on quantitative phase microscopy. It can make up for the deficiency of traditional method and provide a new way to research the dynamical mechanism of firing patterns of neurons.

英文关键词： phase microscopic imaging;neuron;dynamical behavior;firing characterization in phase language;continuous location sampling