构建HFMD-EV71神经损害MR特异性分子探针的实验研究

项目名称： 构建HFMD-EV71神经损害MR特异性分子探针的实验研究

项目编号： No.81460272

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 李建军

作者单位： 海南省人民医院

项目金额： 47万元

中文摘要： 手足口病(HFMD）是儿童常见传染病，肠道病毒71型（EV71）是主要致病病原体之一，具有高度嗜神经特性，感染后病情进展快，易发生严重神经系统并发症，致残率和死亡率高，海南是EV71感染的高发地区。本课题组前期研究结果表明：MRI可显示EV71感染HFMD中枢损害病变的部位和范围，并在一定程度上判断预后，但其发病机制不清，常规MRI检出病变时病程常已进展凶猛。本项目拟构建特异性MR分子探针GoldMag-EV71-VP1-C4抗体探针，通过体外、体内实验，建立人EV71感染食蟹猴动物模型，结合分子生物学技术，阐明EV71选择性嗜神经侵入、靶向性扩散的途径和机制，探讨应用特异性MR分子探针早期诊断EV71感染中枢损害的可能性，以期为临床早期干预治疗，最大程度减轻患儿神经损害提供新方法，具有重要理论意义和临床价值。

中文关键词： 分子探针；EV71病毒；神经损害；磁共振成像；动物实验

英文摘要： Hand, foot and mouth disease (HFMD) is a common infectious disease in children and is charactristed as highly representing philic-nerves, progressing fast after infection, causing severe neurological complications and high morbidity and mortality,enterovirus 71 (EV71) is one of the main pathogen. Hainan is one high-risk areas of EV71 infection. Our preliminary research results showed that MRI can display the lesions site of EV71-HFMD infection and the scope of the damages to the central nervous part, judge prognosis to some extent.But its pathogenesis is not clear, disease progression have often progressed fiercly when conventional MRI detected lesions. This project intends to build specific GoldMag - EV71-VP1-C4 molecular probe of MR in vitro and in vivo experiments of people cynomolgus monkey EV71 infection animal model, combined with the molecular biology technology, tracer EV71 virus proliferation in animal body, histopathological changes and the expression of virus antigen in different organizations, to clarify its selective intrusion, targeted ways to spread, the traceability and descriptive research to further expand as the institutional research.It is expected to seek early accurate diagnosis and reliable inspection methods and specific indicators , for timely and maximizely interventing infection EV71-HFMD to reduce nerve damage and to provide new ideas , which has important theoretical significance and clinical value.

英文关键词： molecular probe;EV71 virus;nerve damage;Magnetic resonance imaging;animal experiment