HIF-1α稳定性在黄芪甲苷促进骨髓间充质干细胞血管新生中的作用机制研究

项目名称： HIF-1α稳定性在黄芪甲苷促进骨髓间充质干细胞血管新生中的作用机制研究

项目编号： No.81202948

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 张玲

作者单位： 浙江大学

项目金额： 23万元

中文摘要： 骨髓间充质干细胞（MSCs）治疗缺血性心脏病是目前干细胞研究领域的热点，然而单独MSCs治疗存在干细胞存活率低及长期疗效并不显著的问题，因此，对MSCs进行修饰和处理是目前研究的难点问题。黄芪甲苷是我国传统中药黄芪的主要活性成分，临床及基础研究显示黄芪甲苷治疗缺血性心脏病疗效显著。本项目前期研究表明黄芪甲苷具有促进MSCs血管新生的作用，但其分子机制还不明确。因此，在前期工作基础上，本项目拟通过体内体外实验评价黄芪甲苷促进MSCs血管新生的作用及抗心肌缺血的保护作用，并以HIF-1α稳定性作为切入点阐明黄芪甲苷促进MSCs血管新生的分子机制。本项目的开展不仅有利于提高MSCs治疗缺血性心脏病的疗效，而且能为扩大传统中药的临床应用范围提供实验依据。

中文关键词： 黄芪甲苷；骨髓间充质干细胞；血管新生；HIF-1α稳定性；心肌修复

英文摘要： Treatment of ischemic heart disease by human marrow-derived mesenchymal stem cells (MSCs) is the hot spots on current stem cell research, however, a separate MSCs treatment exists some problems such as: low cell survival rates and no significance in long-term efficacy. Therefore, modification on MSCs is the difficult problem in current researches. Astragaloside Ⅳ is the main active ingredient of Radix Astragali, a traditional Chinese medicine, clinical and basic studies have shown that Astragaloside Ⅳ exerted an effective treatment on ischemic heart disease. Our preliminary study showed that the Astragaloside Ⅳ promoted angiogenesis of MSCs, but its molecular mechanism is unknown. Therefore, based on our previous work, this project aims to evaluate angiogenesis of MSCs promoted by Astragaloside Ⅳ and protective effect against myocardial ischemia in vitro and in vivo experiments. Furthermore, the HIF-1α stabilization is studied to clarify the molecular mechanisms involved in angiogenesis of MSCs promoted by Astragaloside Ⅳ. The launching of this project will help to improve the efficacy of MSCs treatment on ischemic heart disease, it also provide the experimental basis for expanding the scope of clinical application of Traditional Chinese Medicine

英文关键词： Astragaloside IV；Bone marrow mesenchymal stem cells；Angiogenesis；HIF-1α stabilization；Myocardial repair