重金属胁迫诱导水稻表观遗传变异及胁迫后代产生胁迫适应的分子机制研究

项目名称： 重金属胁迫诱导水稻表观遗传变异及胁迫后代产生胁迫适应的分子机制研究

项目编号： No.31200198

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 微生物学、植物学

项目作者： 欧秀芳

作者单位： 东北师范大学

项目金额： 26万元

中文摘要： 植物表观遗传变异与非生物胁迫的关系是当前研究的热点。我们前期研究发现，重金属胁迫后水稻植株发生了基于DNA甲基化修饰改变的表观遗传变异；而且，受胁迫水稻植株的后代在遗传其亲代所发生的新的DNA甲基化模式的同时，增加了对相应胁迫的适应能力。本项目拟在此基础上，对重金属胁迫诱导产生的表观遗传变异及胁迫植株后代获得胁迫适应的分子机制进行研究；探讨并初步阐明水稻在特定重金属胁迫条件下基因组表观遗传变异与其抗逆性增强之间可能存在的内在关系，期望在水稻中找到一个或几个新的（可能受表观遗传机制调控的）对重金属解毒起重要作用的关键基因。本研究结果预期为进一步理解作物对重金属胁迫的耐受机制以及耐重金属水稻新种质创制提供有价值的参考。

中文关键词： 重金属胁迫；水稻；表观遗传；；

英文摘要： The relationship between epigenetic alteration and adaptive tolerance to abiotic stress has been gaining unprecedented interest in the plant genetics community. We found previously in rice (Oryza sativa) that transgenerationally heritable epigenetic alterations were induced by heavy metal stress, and progenies of the stressed plants showed concomitant inheritance of the altered DNA methylation patterns and enhanced stress tolerance. This proposal is aimed to explore the possible intrinsic links between the two phenomena: inheritance of heavy metal-induced altered epigenetic marker and acquisition of enhanced tolerance to the heavy metal stress. This will be accomplished by identification of one or more functional genes that ply key roles in heavy metal detoxification and that will be regulated by epigenetic mechanisms. Results obtained from this study are expected to advance our understanding on the mechanistic basis of "acquired" heavy metal stress by progenies whose progenitors have experienced the stress, as well as provide useful reference for generating more heavy metal-tolerant (due to enhanced detoxification) crops cultivars.

英文关键词： Heavy metal stress；Rice；Epigenetics；；