褐藻新品种厚叶海带岩藻聚糖硫酸酯的结构分析及其对脂质代谢的影响

项目名称： 褐藻新品种厚叶海带岩藻聚糖硫酸酯的结构分析及其对脂质代谢的影响

项目编号： No.31471610

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 汪秋宽

作者单位： 大连海洋大学

项目金额： 82万元

中文摘要： 项目对褐藻优势新品种厚叶海带（Kjellmaniella crassifolia Miyabe）褐藻多糖硫酸酯(fucoidan)进行结构分析及其对脂质代谢的影响。在气相色谱分析其单糖组成、红外光谱、核磁共振分析其结构的基础上，采用MALDI-TOFMS 和ESIMS/MS分析单糖间及其硫酸根连接结构，比较其结构特征；利用实时-PCR与蛋白印迹法动态检测高脂大鼠肝脏中过氧化酶体增殖物激活受体PPAR-αmRNA的表达及由其调节的肝脏特异性肉碱棕榈酰转移酶(CPT-1)和脂酰辅酶A氧化酶(ACO-1)的活性，动态检测调节元件结合蛋白SREBP-1c变化及其调控的脂肪酸合成酶（FAS）、硬脂酰辅酶A去饱和酶1（SCD-1）的变化；动态检测PPAR-γ及脂蛋白代谢关键酶脂蛋白脂肪酶LPL变化。建立厚叶海带fucoidan的结构理论数据，阐明其对脂肪代谢影响。为厚叶海带培育高效利用奠定基础。

中文关键词： 厚叶海带；褐藻；多糖硫酸酯；结构分析；脂质代谢影响

英文摘要： The studies will be carried out on the structure characteristics and lipometabolic effect for the fucoidan purified from the new brown seaweed of Kjellmaniella crassifolia Miyabe, which contains about 4-5 times of fucoidan compared with that of Laminaria japonica. MALDI-TOFMS and mass-spectrometry ESIMS/MS will be applied for elucidating the complex structural patterns of the monomer composition and their substitution of glycosylation, sulphation and/or O-acetylation based on the monosaccharide composition of the fucoidan analyzed by Gas chromatography and the characteristics of the fucoidan strcuture illustrated by infrared spectrometer and nuclear magnetic resonance. In addition, the structure characteristics of fucoidan from Kjellmaniella crassifolia Miyabe produced in North of Japan will be compared. The changes of peroxisome proliferators activatied receptor PPAR-α , and acyl-CoA oxidase-1 (ACO-1) and carnitine palmitoyltransferase-1 (CPT-1) in lipolytic process modulated by PPAR-α will be detected dynamically by real time transcription and western blotting methods. The major transcription factor involved in the activation of lipogenic genes, mRNA level of SREBP-1c, and that of its two representative target genes, SCD-1 and FAS will be determined at meantime. PPAR-γ,which plays a pivotal role in fat cell differentiation and lipid storage and the key enzyme in lipoprotein and adipocyte metabolism will also be also dynamically analyzed by the method of RT-PCR for detecting the fucoidan acting as dual PPAR agonists. The accomplishment of this project will establish the structure characteristics of fucoidan from Kjellmaniella crassifolia cultured in China and demonstrating its hypolipidemic metabolic mechanism. They will provide scientific base for high efficient utilization of this newly cultured brown seaweed.

英文关键词： Kjellmaniella crassifolia Miyabe;fucoidan;structure characteristics;lipometabolic effect