抗性小麦应答孢囊线虫侵染的ROS相关基因的分离与功能分析

项目名称： 抗性小麦应答孢囊线虫侵染的ROS相关基因的分离与功能分析

项目编号： No.31301645

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 孔令安

作者单位： 中国农业科学院植物保护研究所

项目金额： 23万元

中文摘要： 小麦孢囊线虫（CCN）是世界小麦产区普遍发生、严重威胁小麦生产的病原物。活性氧（ROS）迸发是寄主抵御病原物侵染的机制之一，但就小麦应答CCN侵染中ROS的产生规律尚未见报道。前期工作中，我们从400多份小麦中筛选到高抗CCN的小麦品种VP1620，其单株孢囊数仅为2.4个。本项目拟通过植物病理学、生物化学、生物信息学和分子生物学等方法，研究VP1620和感病温麦19在CCN不同侵染时期产生ROS的种类、产量、ROS形成和消除相关酶的酶活性，以揭示CCN侵染过程中ROS的产生规律。通过挖掘抗感小麦在CCN不同侵染阶段的转录组数据，获得ROS相关的抗性候选基因，并用qRT-PCR、RNAi验证其功能。本项目将揭示ROS在CCN-小麦互作中的产生规律及其与抗性之间的关系，并将为研究其他植物线虫与寄主互作中产生的ROS提供借鉴意义。

中文关键词： 脂氧合酶；第III类过氧化物酶；活性氧；小麦孢囊线虫；

英文摘要： The cereal cyst nematode (CCN) Heterodera avenae is a worldwide distributed pathogen of wheat and other cereal crops, and causes severe economic yield losses. The burst of reactive oxygen species (ROS) is considered as one of important host defense strategies. However, there is no report about generation profiles of ROS during the infection process of CCN on wheat root. In previous studies, we obtained a wheat cultivar VP1620 with high-resistance to CCN by screening more than 400 wheat candidates，and the amount of cysts on VP1620 root was only about 2.4. In this study, we plan to integrate and use the methods of different disciplines, such as plant pathology, biological chemistry, bioinformatics and molecular biology, to decipher the characteristics of ROS, including the category identification, content assay, the respective activities of ROS-production and ROS-scavenger enzymes,in order to clarify the generation profiles of ROS during CCN infection process. We plan to screen and isolate putative ROS-related resistant genes from transcriptomic data of compatible and incompatible reaction between wheat and CCN at different time points, and confirm them by qRT-PCR method, and functional characterize some important candidate genes by RNA interference (RNAi) technique. Our study will clarify the relationship of ROS

英文关键词： Lipoxygenase；Class_III_peroxidase；reactive oxygen species；cereal cyst nematode；