茶叶EGCG通过维护蛋白质内稳态延缓细胞衰老的功能活性与机制研究

项目名称： 茶叶EGCG通过维护蛋白质内稳态延缓细胞衰老的功能活性与机制研究

项目编号： No.31471590

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 蔡淑娴

作者单位： 湖南农业大学

项目金额： 87万元

中文摘要： 随着人口老龄化进程的加速和社会环境的恶化，实现健康的衰老是提升我国人口健康和生活质量、保持经济持续发展的关键。衰老是生化副反应的失修性累积，各种应激容易导致蛋白质错误折叠，促进集聚物的形成，激活衰老基因的表达，加速细胞的衰老。细胞衰老是一个蛋白质内稳态逐渐崩溃的过程。蛋白质内稳态的失修性累积是机体各种衰退性改变的上游生化因子。因此，及时有效维护细胞蛋白质内稳态是实现健康衰老的关键。β-折叠结构是形成蛋白质集聚物的种子结构，茶叶EGCG能够逆转β-折叠结构的形成。本课题组将重点从β-折叠结构破环细胞蛋白质内稳态边界的角度，研究β-折叠结构对泛素化蛋白质集聚物形成和蛋白质泛素降解体系的影响，在此基础上研究EGCG通过维护蛋白质内稳态延缓细胞衰老的作用机制。茶产业是全球21世纪最具发展空间的健康产业之一。本课题将为EGCG的深层次应用开发奠定基础，并将极大提升茶饮在老龄社会中的饮用价值。

中文关键词： 表没食子儿茶素没食子酸酯；细胞衰老；蛋白质内稳态；β-折叠结构

英文摘要： With the accelerated process of population aging and the deterioration of the social environment, the key to sustainable economic development and to improve the health and quality of the population life is to achieve healthy aging.Aging is an accumulation disrepair of biochemical based bypass effects.Various stresses can easily lead to misfolded proteins, which blocks the cells' feedback signal and activates senescent gene expression, accelerating cell aging. The accumulation of steady state of the protein disrepair is the upstream biochemical factos of vary degenerative changes in the body. Therefore, the key to healthy aging is timely and effective maintenance of intracellular protein homeostasis. β-sheet structure is the seed structure for the formation of protein aggregates; EGCG in tea can reverse the formation of β-sheet structure. In this project, we start from the fact that β-sheets structure destroys cell proteostasis boundary, and focus on the effect of β-sheets structure on the ubiquitinated proteins aggregates and the ubiquitin degradation protein system. On this basis, we study the function and mechanism of EGCG delaying cellular senescence by maintaining proteostasis.Tea industry is one of the most promising global health industry for development in the 21st century. This project will lay the solid development foundation for EGCG applications and greatly enhance the value of tea in ageing society.

英文关键词： (-)-epigallocatechine gallate (EGCG);Cell senescence;Proteostasis;β-sheets