Pim-3促进自噬对脓毒血症所致肾小管上皮细胞损伤的保护作用

项目名称： Pim-3促进自噬对脓毒血症所致肾小管上皮细胞损伤的保护作用

项目编号： No.81200544

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学二处

项目作者： 李志莲

作者单位： 广东省人民医院

项目金额： 23万元

中文摘要： 脓毒血症是ICU患者发生急性肾损伤（AKI）的最常见原因。脓毒血症并发AKI死亡率极高，但机制仍不清楚。我们前期的工作发现，脓毒血症动物模型及脂多糖LPS刺激均可触发肾小管上皮细胞（TEC）自噬，且先于细胞凋亡；抗凋亡蛋白丝/苏氨酸激酶家族蛋白Pim-3表达增加，且其抑制剂可减少TEC自噬，促进凋亡，提示pim-3可能通过增强自噬，抑制脓毒血症所致的TEC损伤。本项目拟进一步深入研究：①建立脓毒血症动物和细胞模型，采用雷帕霉素和3-MA分别增强和抑制自噬，获得增强自噬减轻AKI的直接证据。②上调或下调TEC中pim-3水平，观察LPS介导TEC自噬和凋亡的关系，明确pim-3在LPS介导TEC损伤中的保护作用。 ③探讨pim-3调控自噬mTOR依赖途径的可能机制。本课题将从新的角度阐明脓毒血症TEC损伤的机制，及pim-3通过增强自噬减轻AKI的信号通路，为脓毒血症AKI的防治提供新靶点。

中文关键词： 脓毒血症；急性肾损伤；自噬；线粒体；humanin

英文摘要： Sepsis-induced acute kidney injury (AKI) is the most common form of AKI observed in patients in Intensive Care Units(ICUs). Compared with septic patients without AKI,it remains high mortality and worse outcome partly due to our poor outstanding of its pathophysiological mechanisms. Our preliminary observation found that autophagy, as an important survial mechanism, activated in tubular epithelial cells(TECs) when severe sepsis occurred in vivo(cecal ligation and puncture rat model,CLP) and in vitro(LPS stimulation), whereas declined autophagy by 3-MA, an autophagic inhibitor,increased apoptotic TECs, indicating the protective role of autophagy in the pathogenesis of sepsis-induced AKI.Pim-3,a member of serine/threonine kinase family,has been reported to prevent apoptosis and promote cell survival in cancer, inflammatory disorders and ischemic diseases. However,whether its antiapoptotic effect is regulated by autophagy remains unknown.Besides, Pim-3 shares several important substrates with rapamycin, an well-known mTOR inhibtor,indicating Pim-3 play a role in autophagy which is possibly dependent of mTOR. In our study, we in the first time observed the activation of Pim-3 in septic TECs both in vivo and in vitro, and Pim inhibitor SGI-1776 downregulated expression of autophagic proteins such as LCII and Beclin

英文关键词： sepsis；Acute Kidney Injury；autophagy；mitochondrial；humanin