慢病毒介导的RNAi沉默IL-1I型受体联合无热量超短波对鼠关节炎的治疗作用及机制的研究

项目名称： 慢病毒介导的RNAi沉默IL-1I型受体联合无热量超短波对鼠关节炎的治疗作用及机制的研究

项目编号： No.81201503

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学四处

项目作者： 孔瑛

作者单位： 中南大学

项目金额： 24万元

中文摘要： 骨性关节炎是一种常见病，现有治疗仅能延缓其发展。基因治疗是目前研究热点，但受到基因转染率等限制，仅能对其进行实验室研究，何时能让基因治疗尽早进入临床是目前困扰大家的一个难题。脉冲电场能引起细胞膜通透性一过性改变，且是安全、无副作用的。利用这种改变进行基因转染治疗也是研究热点之一。超短波是临床上常用于治疗骨性关节炎的高频脉冲电场，具有穿透性好、无痛、患者服从性高的特点。认为超短波对炎性介质的影响，主要通过非热效应实现。那么超短波非热效应是否与膜通透性的一过性改变有关呢？如果可影响膜的通透性，那么与病毒转染的基因进行联合治疗时，是否可增加转染率呢？有研究显示慢病毒介导的RNAi沉默IL-1Ⅰ型受体能抑制实验性大鼠膝骨关节炎丝裂素原激活的蛋白激酶表达。本实验采用这一成功的转染基因治疗方法联合无热量的超短波来对鼠软骨细胞及骨关节炎的SD大鼠进行干预，尝试证实以上假设，为基因的临床治疗提供一个思路。

中文关键词： 慢病毒；RNA干扰；电穿孔；骨关节炎；超短波

英文摘要： Osteoarthritis is a common existing .The treatments can only slow down the development. Gene therapy is a current research focus, but the gene transfection rate is limit, so the Gene therapy is only in laboratory researc. When to make gene therapy into the clinic as soon as possible is a problem currently plaguing everyone. Pulsed electric field can cause a transient change in membrane permeability, and is safe, no side effects. Take advantage of this change for gene therapy is to study one of the hot. Ultrashort wave is a high-frequency pulsed electric ,and it is commonly used in the treatment of osteoarthritis in the clinical. it has penetrating, painless, so patients obey the characteristics of high. That the impact of ultrashort wave of inflammatory mediators, mainly through the non-thermal effects. Ultrashort wave of non-thermal effects related to changes in membrane permeability over? If you can affect the permeability of the membrane, then combination therapy with virus gene transfection, it increases the transfection efficiency? Studies have shown that lentivirus-mediated RNAi silencing of IL-1 receptor type Ⅰ Suppression of experimental knee osteoarthritis mitogen-activated protein kinase original expression. In this study, the joint success of gene therapy transfection of ultrashort wave of heat to in

英文关键词： lentivirus；RNA interference；electrotransfer；osteoarthritis；ultrashort-wave