近红外光敏多功能纳米载体的构建及药物靶向传递和成像研究

项目名称： 近红外光敏多功能纳米载体的构建及药物靶向传递和成像研究

项目编号： No.21304098

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 陈俊

作者单位： 中国科学院高能物理研究所

项目金额： 25万元

中文摘要： 近红外光刺激响应的纳米材料具有非损伤刺激响应模式，能够精确地控制纳米载体响应的时间和部位，成为癌症诊断治疗新的研究热点。拟以光敏感两亲性聚合物和上转换发光纳米材料为基础，融合两者特点构建新型多功能纳米载体用于提高药物传递的靶向性和可视化。研究不同组份和光学特性的上转换发光纳米材料和紫外可见光降解聚合物的组装过程，及组装体的化学组成和结构与生物相容性的关系。基于从外部近红外光到内部紫外可见光分两步完成的刺激机制，建立聚合物的亲疏水性变化导致纳米颗粒解离的作用模型，研究纳米药物在分子水平、细胞水平和动物体内的光刺激药物释放行为和肿瘤细胞靶向性。同时研究该纳米载体的荧光特性，基于荧光能量转移原理，由纳米载体荧光淬灭到荧光恢复的转变，为靶向肿瘤成像、肿瘤治疗可视化提供基础。此复合材料融合光敏聚合物和无机纳米材料两者优点，具有很好的生物医学应用潜力。

中文关键词： 刺激响应性聚合物；上转换纳米发光材料；近红外光；肿瘤靶向性；肿瘤治疗

英文摘要： Near-infrared (NIR) light responsive nanomaterials take advantage of non-invasive mode, precisely controllable stimulus-responsibility at a specific time and location, which has great potential in the development of a new strategy for cancer therapy and diagnosis. This work proposes a novel strategy to fabricate near-infrared light-responsive multifunctional nanocarrier with core-shell structure, based upon upconversion nanoparticles (UCNP) and light sensitive amphiphilic copolymers (LSP) for improving targeted drug delivery and smart imaging. The self-assembly behavior of the upconversion nanocrystals having a variety of compositions and optical characteristics and UV-vis light sensitive amphiphilic copolymers will be investigated. The toxicity study in vitro and in vivo will used to evaluate the effect of the chemical compositions and structure on the biocompatibility of the nanocarriers. Based on the mechanism of two-stepped stimulus from external NIR light to internal UV-vis light, the work will establish the model of enabling the use of NIR light to triggered disrupt the nanocarriers and triggered release their "payloads", in which the NIR light is upconverted to UV-vis light by upconversion nanoparticles to degrade the copolymers. The in vitro and in vivo NIR light-triggered drug release profiles, tumor-ta

英文关键词： stimuli-responsive polymer；up-conversion nanoparticle；near-infrared light；tumor-targeting；tumor therapy