基于无酶循环放大策略的多通道纳米电化学生物传感器用于肝癌多元肿瘤标志物的联合检测

项目名称： 基于无酶循环放大策略的多通道纳米电化学生物传感器用于肝癌多元肿瘤标志物的联合检测

项目编号： No.21205015

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 分析化学

项目作者： 张静

作者单位： 福建农林大学

项目金额： 23万元

中文摘要： 我国是肝癌的重灾区，发病率呈逐年上升趋势。因初期症状不明显，肝癌确诊时已多属中、晚期，缺乏有效治疗。因此，肝癌早期诊断极为重要。然而目前临床上常用的影像学检查和肝组织活检等方法或灵敏度低、昂贵、耗时，或操作复杂、创伤性大。因此，研究一种灵敏、准确、简便、快速、经济、非创伤性的检测新技术，对提高肝癌早期诊断的准确率和开展全国范围内尤其是经济欠发达地区的肝癌普查，具有极其重要的意义。因此，本项目拟研制一种基于"无酶循环信号放大策略"的多通道纳米电化学生物传感器，并将其用于DNA、microRNA和生长因子三种不同类型肝癌标志物的联合检测。通过对比肝癌组、良性肝病组与健康对照组血液中标志物的水平，建立肝癌早期诊断的新方法。与目前临床上使用的基于甲胎蛋白单一肿瘤标志物的检测技术，或基于抗原、酶、蛋白质等联合检测的蛋白芯片技术相比，该传感器可联合检测多元肿瘤标志物，大大提高了肝癌早期诊断的准确率。

中文关键词： 肝癌；肿瘤标志物；DNA电化学传感器；信号放大策略；

英文摘要： Liver cancer is one of the common malignant tumors in China, with the morbidity rising year by year. A significant proportion of liver cancers initially are asymptomatic lesions and are not diagnosed or treated until they reach an intermediate or advanced stage. Therefore, the early diagnosis of liver cancer is the most effective means to reduce mortality rate from this disease. So far, the commonly used early diagnosis are imageology and hepatic tissue biopsy in clinic. However, these methods have some disadvantages including lower sensitivity, higher assay costs, relatively long assay times, more complicated operation, and invasion. Thus, it is very important to develop a kind of sensitive, accurate, convenient, fast, economic, non-invasive method for the increase of accurate rate and the implementation of nationwide large scale screening. In this research project, we will develop a multichannel nano-electrochemical biosensor based on enzyme-free recycling. By employed this sensor, the combined detection for three kinds of tumor markers such as DNA, microRNA and growth factor will be realized. By comparing the levels of tumor markers between liver cancer group, benign liver disease group and healthy control group, we can establish a new method for early diagnosis of liver cancer. Compared with the current use

英文关键词： Hepatocellular carcinoma；Tumor marker；DNA electrochemical sensor；Signal amplification strategy；