新型高灵敏近红外SERS探针合成及其活体多元成像应用研究

项目名称： 新型高灵敏近红外SERS探针合成及其活体多元成像应用研究

项目编号： No.21305157

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 王文海

作者单位： 中国科学院烟台海岸带研究所

项目金额： 25万元

中文摘要： 表面增强拉曼散射 (SERS) 探针具有高灵敏度、高空间分辨率和多元标记等优势，在活体拉曼成像领域引起了极大关注。目前SERS探针研究主要集中于贵金属纳米基底，对其光学品质同样具有重要影响的拉曼报告分子(RR)的开发则严重滞后。本研究拟解决近红外（NIR）SERS探针制备中RR种类局限的根本问题，从SERS机理、探针组装和活体应用所需求的化学结构出发，利用组合化学手段，设计、合成和筛选基于方酸菁和花菁两类荧光染料母核的全新RR，开发具有多种光谱指纹特征、适用于活体多元标记的NIR SERS探针组合。进一步实现对不同电荷脂质体模型纳米药物的标记。通过解析活体小鼠特征组织部位各SERS探针的特征峰信号，实现不同靶向性质脂质体的体内同时监测，进一步获取其体内药动学参数信息。课题的实现将极大提升SERS探针的光学品质，推动拉曼活体成像技术向高通量筛选和实际应用方向发展。

中文关键词： 表面增强拉曼散射；荧光；光学探针；脂质体；光学成像

英文摘要： As novel imaging technique and optical nanoprobe,Raman in vivo imaging technique and surface-enhanced Raman scattering (SERS) tags draw a lot of attention owing to the high sensitivity, high spatial resolution and multiplex labeling ability. So far, the study on SERS tags has been mainly focused on the noble metal nanosubstrate. However, the development of Raman reporter, which is another key factor affect the optical quality of SERS tags, is far lagged behind. In this program, we aim to resolve the problem of limit of types and random selection of Raman reporter during the SERS tag synthesis.Novel near infrared (NIR) reporters that suitable for in vivo SERS tag will be designed and screened. Libraries of molecules will be synthesized based on NIR dye core structures of squaraine and cyanine with the aid of combinational chemical synthesis technique, and subject to further reporter screening and preparation of NIR SERS tags with various fingerprt spectra for multiplex in vivo Raman imaging. Furthermore, liposomes with different surface charges will be labeled with SERS tags with various optical fingerprints. Taking advantages of laser Raman system, it will be realized that the simultaneous detection of various types liposomes in tumor node mouse by analyzing the characteristic Raman information of each tag. The

英文关键词： Surface-enhanced Raman scattering；Fluorescence；Optical probes；Liposome；Optical imaging