柿果实乙烯合成的启动与氧化还原电位及相关酶活性的关系

项目名称： 柿果实乙烯合成的启动与氧化还原电位及相关酶活性的关系

项目编号： No.31501809

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 孙宁静

作者单位： 广西大学

项目金额： 20万元

中文摘要： 柿是我国的特色水果，具有广阔的国际市场，但采后储藏问题严重阻碍柿产业的发展。控制乙烯代谢对于延长果实储藏期具有重要意义，但乙烯生物合成的启动机制尚不清楚。申请人前期研究发现柿树体内动态的“光合产物流”对果实乙烯合成启动起到抑制作用。据此提出假设：树体“光合产物流”影响果实乙烯合成启动的实质是通过维持“高氧化还原电位”阻碍ACC氧化为乙烯。本项目拟采用采前/采后不同处理，研究对果实乙烯释放的影响，明确果实乙烯合成的启动与氧化还原电位及相关酶活性的关系，确定参与柿果实乙烯启动的关键酶并研究其编码基因的表达，阐述采前/采后柿果实乙烯合成的启动机制，建立统一的生物模型，为乙烯生物调控机理研究积累基础性资料，也为果实保鲜期的调控提供理论依据。

中文关键词： 柿；乙烯；启动；氧化还原电位；相关酶

英文摘要： As a featuring characteristic fruit of China, persimmon (Diospyros kaki) has a wide international market, while the postharvest problems retard its industry development. Regulation of ethylene is meaningful to prolonging the fruit storage period, but the mechanism of induction of ethylene synthesis in fruit is still unknown. Our previous study suggest that on-tree transport of photosynthates to fruit inhibits the induction of ethylene synthesis in persimmon, and it is hypothesized that the sugar supply would keep the oxidation-reduction potential (ORP) at a high level so ACC could be not oxidized to ethylene. The present project will apply different pre-/post-harvest treatments to investigate their effect on fruit ethylene production, in order to the find relationship among ethylene induction, fruit ORP and related enzymes activity, also to characterize the key enzymes and their coding gene expression. The mechanism of ethylene induction in pre-/post-harvested fruit will be clarified and a unified biological model will be established. New evidence for study on ethylene regulation and guidance for improving fruit storability will be provided.

英文关键词： persimmon;ethylene;induction;oxidation-reduction potential (ORP);related enzymes