拓扑异构酶II-alpha在脊髓损伤后活化星形胶质细胞干细胞特性获得和维持中的作用及机制研究

项目名称： 拓扑异构酶II-alpha在脊髓损伤后活化星形胶质细胞干细胞特性获得和维持中的作用及机制研究

项目编号： No.31301210

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 袁一旻

作者单位： 中国人民解放军第二军医大学

项目金额： 26万元

中文摘要： 脊髓损伤是神经损伤修复领域中的难点和重点，目前还没有有效的治疗手段。干细胞治疗被认为是一个重要的发展方向，尤其是内源性干细胞的研究和运用。脊髓损伤后的局部微环境中胶质细胞活化，尤其是星形胶质细胞的活化是一个重要的生物学现象。活化的星形胶质细胞中有一类亚群被证实具有某些神经干细胞或前体细胞的特性，但目前对这类细胞特性的相关研究甚少。为了寻找参与起始和维持脊髓损伤后活化星形胶质干细胞特性的重要分子，我们分选正常和损伤GFAP-GFP小鼠脊髓组织GFP标记的细胞，通过基因芯片的差异比对筛选，发现拓扑异构酶II-alpha特异表达在脊髓损伤后Gfap、Nestin阳性的细胞中。本研究将综合运用细胞生物学、分子生物学和形态学等技术手段，进一步深入研究拓扑异构酶II-alpha在干细胞样活化星形胶质细胞中自我更新和多能性维持的作用，以期为应用内源性干细胞治疗脊髓损伤提供新的思路和理论依据。

中文关键词： 胶质细胞；拓扑异构酶II-alpha；干细胞；；

英文摘要： Spinal cord injury (SCI) is the difficulty and focus in the field of nerve injury and repair,so far,there is no substantive treatment. Stem cell therapy is considered to be an important direction of development, especially the endogenous stem cell research and use. Glial cell activation(gliosis)after spinal cord injury in the local microenvironment, especially astrocytes activation (astrogliosis) is an important biological phenomenon. One subset of activated astrocytes has been confirmed to have the characteristics of the neural stem cells or precursor cells, but there is very little research on the properties of these cells. In order to find the important molecules involved in the initializing and maintaining the stem cell properties of reactive astrocytes in the injuried spinal cord, with sorting GFP-positive cells from normal and injury GFAP-GFP mice around the spinal cord and gene chip screening,we discovered and confirmed that the topoisomerase II-alpha is specifically expressed in Gfap and Nestin positive cells localized in the injuried spinal cord. In this study, we integrated use of technical means of cell biology, molecular biology, and morphology, and further in-depth research topoisomerase II-alpha in activated astrocytes in self-renewal and pluripotency of stem cell properties, to provide important

英文关键词： glial cell；topoisomerase II-alpha；stem cell；；