大西洋鲑在密度胁迫下的生长免疫代偿功能机制研究

项目名称： 大西洋鲑在密度胁迫下的生长免疫代偿功能机制研究

项目编号： No.31402314

项目类型： 青年科学基金项目

立项/批准年度： 2015

项目学科： 畜牧学与草地科学、兽医学、水产学

项目作者： 王彦丰

作者单位： 中国科学院海洋研究所

项目金额： 10万元

中文摘要： 密度胁迫引起的鱼类应激反应是导致养殖鱼类对病原敏感性增高，群体生长率和存活率下降的重要原因。本研究以大西洋鲑为研究对象，封闭循环水系统养殖密度为研究条件，分析养殖密度引起封闭循环水养殖大西洋鲑应激状态的临界点，筛选适合描述大西洋鲑应激状态的生理生化参数和分子生物标记物，揭示密度胁迫状态下大西洋鲑生长状态与非特异性免疫因子的关系，探讨在密度胁迫状态作用下大西洋鲑的生理响应和免疫应答规律；并研究分析下丘脑-垂体-甲状腺-肝脏轴与大西洋鲑生长和非特异性免疫的关系以及作用机制，以期从组织学、分子生物学、发育生物学并结合内分泌通路系统阐明养殖密度对大西洋鲑生长和抗病力的影响，完善环境因子对鱼类生殖发育的调控机制，掌握封闭循环水系统养殖大西洋鲑鱼的生态数据，优化国内封闭循环水系统生产性能。

中文关键词： 养殖密度；大西洋鲑；生长；生理响应；封闭循环水

英文摘要： The fish stress response caused by density stress is a major cause of farmed fish increasing susceptibility to pathogens and decline of the survival rate.In this study,we take the Atlantic salmon as the research object,breeding density and the closed circulating water system as the research conditions,analyze the critical point of the closed circulating water farmed Atlantic salmon’s stress state caused by breeding density,screen the molecular biomarkers and parameters of the physiological and biochemical which suitable to describe the stress state of Atlantic salmon,reveal the relationship between Atlantic salmon growth state and non-specific immune factors under stress state density,discuss the physiological response and low of immune response of Atlantic salmon under the action of density stress.At the same time,we Research and analysis the relationship between the hypothalamus - pituitary - thyroid - liver shaft and Atlantic salmon growth and nonspecific immune as well as the mechanism of action,In order from histology, molecular biology, developmental biology and endocrine pathway system to illustrate the breeding density effects on growth and disease resistance of the Atlantic salmon,improve the regulatory mechanism of environmental factors on fish reproductive development,master the ecological data of th

英文关键词： stocking density；Atlantic salmon；growth；physiological response；recirculating aquaculture system