基于蛋白金属编码的适体夹心法对多真菌毒素的快速电化学分析研究

项目名称： 基于蛋白金属编码的适体夹心法对多真菌毒素的快速电化学分析研究

项目编号： No.31501557

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 樊凯

作者单位： 杭州电子科技大学

项目金额： 21万元

中文摘要： 真菌毒素对于人体和动物都具有致癌、致畸、致突变等毒害作用，其中黄曲霉毒素B1（AFB1）与赭曲霉毒素A(OTA)的毒性最大。研究简便、灵敏、快速的真菌毒素自动化检测技术对于监控真菌毒素、加强食品安全保障有着重大的现实意义。本项目提出以石墨烯-纳米金复合物修饰的去铁铁蛋白金属分子作为标记物，结合修饰有适配体的磁珠作为探针的电化学夹心体系用于AFB1与OTA的同时检测。拟采用磁珠作为固相载体，核酸适配体进行剪切分为两段作为夹心体系的两组核酸片段，重点提高小分子的捕获效率和检测灵敏度。最后同步检测农贸市场抽检的实际样品，并与官方标准方法比较结果，力求为我国研制足有自主知识产权的真菌毒素检测技术和仪器提供新思路。

中文关键词： 适配体；毒素；标记；磁珠

英文摘要： The consumption of mycotoxins-contaminated diet is potentially a health hazards for both humans and animals through induction of acute and chronic effects that have teratogenic, carcinogenic or immunosuppressive impact. Among mycotoxins of gtretest public health and agroeconomic significance are aflatoxins B1(AFB1) and ochratoxin A(OTA). Research on easy to use, sensitive, rapid and automated detection technology for mycotoxins is of great practical significance to monitor the mycotoxins and strengthen the food safety. This investigation is carried out for rapid and simutaneous determination of the AFB1 and OTA levels in food using graphene-Au grafted recombinant apoferritin-encoded metallic labels as signal tags and dual-aptemer magnetic beads as capture probes. We intend to focus on enhancing the capture efficiency and the resulting sensitivity of the proposed assay, by taking the magnetic beads(MBs) as the solid phase and splitting the aptamer into two nucleic acid sequence to construct the sandwich system. Finally, the proposed assay will be further evaluated through collaborative detection of the same practical sample using the official analysis method of HPLC or HPLC-MS. Hopefully, the project may provide new ideas and help pave the way for the R&D of Chinese detection technology and instrument for mycotoxins with independent intellectual property.

英文关键词： aptamer;mycotoxins;lables;magnetic beads