海洋放线菌ACMA006抗肿瘤活性物质抑制肝癌的实验研究

项目名称： 海洋放线菌ACMA006抗肿瘤活性物质抑制肝癌的实验研究

项目编号： No.31300103

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 袁献温

作者单位： 南京大学

项目金额： 23万元

中文摘要： 从96株海洋微生物中筛选得到1株具有较强抗肿瘤活性的菌株，采用硅胶柱层析，薄层层析及HPLC等方法对抗肿瘤活性物质进行分离鉴定；通过MTT，荧光实验及流式细胞术对活性物质体外抗肿瘤活性进行测定，建立小鼠肝癌模型，通过体内注射活性物质对其体内抗肿瘤活性进行测定，采用基因芯片及蛋白质组学的方法对活性物质的抗肿瘤机制进行研究。

中文关键词： 肝细胞癌；海洋放线菌；泛素特异性蛋白酶39；；

英文摘要： 1 strain with antitumor activity were screened from 96 strains of marine microorganisms,which had strong antitumor activity, and its cytotoxicity to normal cells was small，the anti-tumor materials were identified and isolated from the fermentation broth of the strain by chromatography and spectral analysis; the anti-tumor activity in vitro was determined by MTT,fluorescence experiments and FCM, hepatocarcinoma mouse model was successfully established,the anti-tumor activity in vivo was confirmed by treatment on the tumor-bearing mouse, study the mechanisms of its anti-tumor activity by gene chip and proteomics.

英文关键词： Hepatocellular carcinoma；marine actinomycete；USP39；；