艾灸抗衰老疗效维持的DNA甲基化调节机制及时效关系研究

项目名称： 艾灸抗衰老疗效维持的DNA甲基化调节机制及时效关系研究

项目编号： No.81202745

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 宁友

作者单位： 复旦大学

项目金额： 23万元

中文摘要： 艾灸抗衰老作用明显，远期疗效持久，即撤出艾灸干预后，抗衰老效应仍然持续，但其疗效的维持机制尚不清楚，值得深入研究。表观遗传修饰可使基因表达沉默或激活，这种修饰一旦形成，即使停止干预，也能长时间维持。DNA甲基化是表观遗传的主要修饰手段，与衰老关系密切。本项目利用甲基化修饰在解析疗效维持方面的特性，对艾灸抗衰老效应的维持机制进行深入研究。 在前期工作基础上，本项目运用艾灸干预自然衰老小鼠，动态观测撤灸即刻到灸后90天内小鼠神经肌肉协调性及学习能力；并取下丘脑等组织，用全基因组甲基化技术筛选被艾灸显著调节而在撤灸后甲基化维持不变的基因，运用Dynamic-GeneNet等生物信息学工具对挑选基因进行分析，找到基因动态变化过程中的核心共表达基因；并从组织、蛋白、基因三个层面进行深入地时效关系变化研究。为临床合理及有效地运用艾灸进行抗衰延寿、提升生存质量提供实验依据和相应的分子标志物。

中文关键词： 艾灸；衰老；疗效维持；DNA甲基化；

英文摘要： Moxibustion has a significant anti-aging effect. Numerous researches indicated that the anti-aging function of moxibustion could not only take effect immediately, but also has a good long-term efficacy. It means that after withdrawal of moxibustion intervention, the effect still continues. However, the underlying mechanism is still unclear, which is worthy for further study. Epigenetic modification of genomic DNA could achieve the effects of gene silence or activation without changing the sequence. Once the modification was formed, the effect could last for a long time even if the intervention was stopped. DNA methylation is one of the most important types of epigenetic phenomenon, which is closely related with senescence. Based on the characteristics of good demonstration for persistent effect of DNA methylation, the study tries to investigate the maintenance mechanism of moxibustion. On the basis of preliminary work, by using the natural aging mouse model, the study will observe dynamically the aging-related neuromuscular coordination and the learning ability of mice at the time point of 0, 7, 30, 60 and 90 day after moxibustion intervention. The tissue samples, such as hypothalamus, will be used to screen the genes that the methylation level was regulated by the moxibustion but unchanged after withdrawal. A

英文关键词： Moxibustion；aging；maintenance of effect；DNA methylation；