项目名称: 质谱分析用于在线监测溶液化学反应的方法学研究
项目编号: No.21205111
项目类型: 青年科学基金项目
立项/批准年度: 2013
项目学科: 分析化学
项目作者: 黄光明
作者单位: 中国科学技术大学
项目金额: 28万元
中文摘要: 要实现对溶液中的化学反应的在线监控、捕捉反应中间体,需要满足 高选择性,高灵敏,高速度的要求,对分析方法学是一个严峻的挑战。本项目拟设计和建立质谱分析在线监测溶液化学反应的方法学研究,拟将其应用于溶液中化学反应的实时在线监测,构建新型化学反应监测方法。通过对时间分辨质谱分析方法的研究,研究脉冲电压的各个参数对于感应电喷雾的影响规律,探索感应纳升电喷雾的时间分辨极限。在此基础上,建立化学反应的直接在线及流动注射监测,通过实时检测化学反应产物的种类和浓度,达到在线检测化学反应进程的目的。同时,本项目还将进行检测低浓度的反应中间产物的研究,有望捕捉到寿命在毫秒量级的亚稳态反应中间体,并确定其结构信息,从而为化学反应的机理研究提供实验基础。本研究方案简单可靠,同时具有高灵敏度和高选择性的特性,有益于自动化和商业化,在化学反应理论研究以及药物合成路线筛选,都具有广阔的理论和实际应用前景。
中文关键词: 质谱分析;在线监测;化学反应;反应中间体;
英文摘要: Online monitoring method for solution phase chemical reactions requires high selectivity, high sensitivity and high speed, it remains a great challenge for analytical chemistry. This proposal is to aiming at developing online monitoring method, which is based on mass spectrometry. Proposed investigation includes further mechanism study of electrospray mass spectrometry, study about the relationship between applied pulsation and each single spray pulsation and time resolution of the inductive nanoelectrospray. Based upon above research, inductive nanoelectrospray is to be coupled with chemistry reaction monitoring directly or with flow injection system to measure the reaction products. Furthermore, the proposal is also aiming at capture and identification of reaction intermediate, providing experimental proof for possible reaction mechanism study. The proposed approach is easy and reliable with high sensitivity and selectivity. It can be easily automated and also commercialized, shows great potential on both chemistry reaction mechanism study or monitoring of pharmaceutical synthesis.
英文关键词: mass spectrometry;online monitoring;chemical reaction;reactive intermediate;