基于网络建模解析水稻在不同非生物逆境下miRNA 的串扰调控关系

项目名称： 基于网络建模解析水稻在不同非生物逆境下miRNA 的串扰调控关系

项目编号： No.31301248

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 张世华

作者单位： 安徽农业大学

项目金额： 23万元

中文摘要： 低温、干旱等非生物逆境严重影响水稻产量和品质。现有研究表明，miRNA在水稻对单一非生物逆境的应答中起着网络化的调控作用，但不同逆境下miRNA调控网络之间的串扰关系尚不明确。本研究拟采用文献挖掘方法，从PubMed和Scopus文献数据库中构建水稻逆境-miRNA-靶基因之间的非线性关联图谱，据此建立水稻非生物逆境下特异的miRNA-gene调控网络，以挖掘不同逆境下miRNA调控基因网络化作用模式上的串扰关系；结合TAIR水稻通路数据库，对不同逆境下miRNA所介导的应答通路之间的串扰互作进行识别；对所获预测结果，选取代表性的非生物逆境组合，利用荧光定量PCR进行验证。本项目的顺利实施将对揭示水稻不同非生物逆境下miRNA对下游基因或通路的串扰调控关系，挖掘抗逆关键基因及利用基因工程进行良种选育具有重要理论指导意义。

中文关键词： 水稻；非生物逆境；miRNA；转录后调控；生物信息学

英文摘要： Abiotic stress (e.g., low temperature, drought etc.) seriously affect the yield and quality of rice. It has been demonstrated that miRNA behaves network regulatory role in rice against a certain abiotic stress, however, miRNA regulatory crosstalk relation for rice under different abiotic stress conditions remains not clear. In this project，we will construct the non-linear relationship profile of stress-miRNA-target gene in rice from PubMed and Scopus literature databases, using literature mining method. On this basis, we will 1)establish stress-specific miRNA-gene regulatory network for rice to detect crosstalk relation of miRNA-gene network regulation mode; 2) mining the crosstalk interaction among miRNA-mediated responsible pathways under different abiotic stress conditions, by using TAIR rice pathway database, and 3) choose representative abiotic stress combination, to verify the above computational prediction with fluorogenic quantitative PCR. The implementation of the present project will have important theoretical guiding significance in exploring the regulatory crosstalk relation of the interaction of miRNA and downstream genes (pathways) under different abiotic stress conditions, digging key stress-resistant genes, and the application in improved variety breeding with genetic engineering for rice.

英文关键词： rice；abiotic stress；miRNA；transcriptional regulation；bioinformatics