脐带Wharton胶来源的仿生化支架/干细胞复合构建组织工程神经及其体内外生物学评价

项目名称： 脐带Wharton胶来源的仿生化支架/干细胞复合构建组织工程神经及其体内外生物学评价

项目编号： No.81201212

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 影像医学与生物医学工程

项目作者： 郑希福

作者单位： 大连医科大学

项目金额： 23万元

中文摘要： 包括自体神经移植在内的各种方法应用于周围神经损伤修复，但神经功能恢复效果仍然不理想。组织工程神经技术为解决该难题提供了可能，但目前仍然存在急需解决的难题：研发新型支架材料、支架成份及结构的仿生化设计、干细胞向神经细胞的有效诱导及准确检测等。课题组拟针对以上难题,结合前期微流控芯片、脱细胞及仿生化定向支架制备技术，进行以下探索性研究：脐带中提取脐带间充质干细胞（UCMSCs）及脐带Wharton胶；通过新型微流控芯片技术，筛选bFGF联合PDGF诱导UCMSCs向雪旺细胞转化的最佳诱导浓度；Wharton胶脱细胞获得细胞外基质材料，并利用其制备仿生化定向结构的神经导管组织工程支架，对其结构及性质进行体内外生物学评价；将诱导转化的UCMSCs种植于支架，评价细胞亲和性、排列及功能情况。探讨其在神经组织工程应用的可行性。为进一步神经修复动物实验及临床应用提供理论与技术基础。

中文关键词： 脐带间充质干细胞；脐带Wharton 胶；微流控芯片；仿生化定向支架；神经再生

英文摘要： Although various kinds of methods including autologous nerve grafting were available for the peripheral nerve lesions repair. Unfortunately, the result of nerve function recovery was still undesirable. The tissue engineering nerve technique has provided a promising alternative for nerve repair. However, there are many problems which should be resolved as soon as possibly: a new type scaffold biomaterial should be researched and developed; the never scaffold was designed with biomimetic chemical composition and microstructure; the stem cell was induced effectively to neural cell and induction result should be detected exactly. To aim directly at above-mentioned difficulties and according to related researches of microfluidi、decellularization and fabrication of oriented scaffold，our research team plan to make a exploratory study as follows: the umbilical cord mesenchymal stem cells (UCMSCs) and Wharton's jelly were got out from umbilical cord; the optimal induction concentration of UCMSCs being induced into Schwann cell by bFGF and PDGF was detected by Microfluidic Technology; the ECM origined from decellularized Wharton's jelly was fabricated into tissue engineering nerve conduit scaffold with biomimetic orientation microchannel structure. The microstructure and property of nerve conduit was assessed biologically

英文关键词： the umbilical cord mesenchymal stem cells；Wharton's jelly；Microfluidic；biomimetic orientation scaffold；nerve regeneration